


Abstract:During‘The 14th Five-year Plan’period,China has vigorously promoted various tasks in the seed industry,marking a crucial stage for the high-quality development of pepper genetic breeding.Although research on pepper genetics and breeding was not independently funded as a separate project in the national key R & D programs,it has received support from multiple scientific and technological projects of relevant national authorities,including programs for precise germplasm identification,gene discovery,germplasm innovation,and rural revitalization.Driven by genomics and multi-omics technologies,substantial progress has been made in both the genetic mechanisms and breeding technologies of pepper,representing one of the fastest-developing phases in recent years.There has been a significant increase in fundamental theoretical research,as well as in the diversity and number of released varieties,accompanied by an elevated proportion of high-quality cultivars.This paper 14th Five-year Plan’period,and provides an outlook for development in‘The 15th Five-year Plan’.
Abstract:This review summarizes recent research advances in efficient haploid breeding for field corn and fresh corn.We focus on the development of haploid inducer lines,haploid identification,and chromosome doubling,and systematically elucidate the effects of induction lines,maternal genetic materials,ecological and climatic conditions,and pollination periods on haploid induction efficiency in fresh corn.The findings provide theoretical guidance and practical application references for haploid breeding in fresh corn.
Abstract:ProT is a type of amino acid transporter located on the plasma membrane,belonging to the AAAP family,responsible for the transport of amino acids from the apoplast to the cytosol.This experiment cloned and performed bioinformatics analysis and localization analysis on the protein encoded by SlProT1.The results showed that the SlProT1 encodes 441 amino acids,with a molecular weight of 48.54 Kda and a theoretical isoelectric point of 9.34.It is a stable hydrophobic protein with a protein structure including alpha helices,random coils,and extended strands.Real time fluorescence quantitative analysis showed that the SlProT1 is expressed in the root,stem,leaf,and flower tissues of tomatoes,with the highest expression level in the leaves,significantly higher than in the roots,stems,and flowers.Bioinformatics prediction shows that SlProT1 may be located on the plasma membrane and vacuolar membrane,while subcellular localization of SlProT1-mCherry comfirmed that SlProT1 is located on the plasma
membrane.
Abstract:Fruit pericarp thickness in pepper is closely associated with cell wall components and xyloglucan
endotransglucosylase/hydrolase(XTH)enzyme activity,yet relevant research remains insufficient.This study measured the contents of cellulose,hemicellulose,and pectin,as well as XTH activity,in pericarp tissues of five pepper inbred lines(three thick-pericarp pepper and two thin-pericarp pepper)at 10 and 35 days after pollination(DAP),and performed comparative and correlation analyses.The results showed that principal component analysis(PCA)indicated the first three principal components cumulatively explained 95.39% of the variance,revealing significant differences in metabolite accumulation patterns between the two pepper types.Quantitative analysis demonstrated that total pectin,cellulose,and hemicellulose contents were all higher in thin-pericarp peppers than in thick-pericarp peppers,and the contents of these three components were generally lower at 35 DAP compared to 10 DAP across the five pepper lines.Conversely,XTH activity was relatively higher in thick-pericarp peppers and was generally elevated at 35 DAP.Further correlation analysis revealed significant positive correlations among pectin,cellulose,and hemicellulose,while showing negative correlations with XTH activity.These findings indicate that cultivar type differences are important factors causing variations in cell wall components and XTH activity,providing a theoretical basis for improving pepper fruit quality through the regulation of cell wall homeostasis.