MOU Hailin, ZHU Hongjiang, LONG Cha, JIANG Suyan, LANG Yunlong, WANG Renxian, CAI Xingyu, LONG Zhengxiang, ZHAO Junjie, DU Zhongguo, YANG Fupeng, LYU Jinli, HU Juan, ZHU Shouliang, LI Wei
Calcium-dependent protein kinase（CDPK）are key regulators of stress signal transduction in
plants and participate in responses to drought and other abiotic stresses．However，systematic identification
and drought response mechanisms of the CDPK gene family in ginger（Zingiber officinale R.）remain largely
unclear．In this study，using‘Zhenning Erhuang ginger’as material，genome-wide identification and
bioinformatics analysis were performed to characterize the ZoCDPK gene family，including gene identification，
chromosomal localization and structural analysis．Combined with drought stress treatment，physiological index
detection and qRT-PCR，the drought response patterns of ZoCDPK genes were further investigated．The results
showed that 57 ZoCDPK members were identified from the ginger genome，which were unevenly distributed
on 16 chromosomes and highly conserved in structure．Based on phylogenetic relationships and promoter ciselement
analysis，ZoCDPK10，ZoCDPK19 and ZoCDPK47 were screened as key candidate genes in response
to drought stress．Under drought stress，the photosynthetic system of ginger was damaged，chlorophyll content
and photosynthetic parameters decreased significantly，while water use efficiency increased．qRT-PCR analysis
revealed that all three candidate genes were up-regulated by drought induction：ZoCDPK19 and ZoCDPK47
showed continuous up-regulation，whereas ZoCDPK10 exhibited an early response pattern with an initial
increase followed by a decrease．This study clarifies the basic characteristics and drought response patterns of the
CDPK gene family in ginger，providing important gene resources and theoretical foundations for dissecting the
molecular mechanisms of drought resistance and facilitating molecular breeding in ginger.
