To explore the potential application of ethanol extracts from Camellia oleifera cake in controlling Meloidogyne incognita, laboratory toxicity assays, egg hatching inhibition tests, mixture synergy experiments, and pot control efficacy trials were conducted. Focusing on the ethanol extract alone and its combination with fluopyram 41.7% suspension concentrate (SC), the nematicidal and inhibitory activities against M. incognita were systematically evaluated, and the synergistic effect of the combination as well as its pot control efficacy were analyzed. The ethanol extract exhibited significant nematicidal activity against M. incognita second-stage juveniles (J2s) , with the EC50 value decreasing over time and reaching 1.43 mg · mL-1 at 72 h. The extract also strongly inhibited egg hatching, showed an inhibition rate of 85.12% after 7 days at 15.0 mg · mL-1. Synergism analysis revealed that combinations of fluopyram and the ethanol extract at toxic unit (TU) ratios of 1∶1, 1∶3, 1∶5, 1∶9 and 3∶1, all combination index (CI) < 1, and the synergy ratios (SR) were all greater than 1, indicating significant synergistic effects. In pot experiments, the 1∶5 mixture treatment achieved 85.41% efficacy in reducing rhizosphere J2s populations and 82.06% in reducing root gall, both obviously superior to single-agent treatments. In conclusion, the ethanol extract of C. oleifera cake exhibited strong biological activity against M. incognita and showed a synergistic effect with fluopyram. The combined application
obviously enhanced control efficacy at appropriate ratios, providing a scientific and technical basis for the green management of root-knot nematodes in protected vegetable production using C. oleifera by-products.