ZHANG Yunyang, YUE Shizhong1, 3, 4, WANG Junru1, 2, ZHAO Xingtang2, 3, 4, WANG Ruiping1, 3, 4, LI Huaitao5
The popularity of selenium（Se）enriched sprouts is increasing due to their high nutritional
value and unique flavor．However，the impact of Se supplementation on the nutritional elements of seedlings
has received minimal attention to date．The experiment involved three Se concentrations（2.0，1.0，and
0.5 mg · L-1），with the control group（CK）receiving no Se supplementation．The experiment aimed to
investigate the effects of sodium selenite（Na2SeO3）on the growth of pea seedlings，as well as its impact on Se
accumulation and the dynamics of nine essential nutrients（K，Ca，Mg，Fe，Zn，B，Mn，Cu，and Ni）．
The findings indicated that，in comparison with CK group，treatments of 0.5 and 1.0 mg · L-1 Se enhanced the
fresh quality of pea seedlings，while 2.0 mg · L-1 Se treatment inhibited fresh quality of seedlings and roots．
On the 10th day after germination，the contents of Se in sprouts and roots reached maximum of 9.12 μg · g-1
and 405.86 μg · g-1（2.0 mg · L-1 Se treatment），respectively．Furthermore，in treatments with the same Se
concentration，the root Se content is significantly higher than that of sprout，and the root has a greater ability
to accumulate Se than the shoot．Biotransport factor of Se（BTFSe） of the samples was all found to be less than
1.00，and it was inhibited by increased culture duration．On the 10th day after germination，the proportion of
organic Se in the shoots was found to be over 95%，indicating its capability for robust organic Se conversion．
The accumulation of nine elements in pea sprouts and their root-to-shoot transport exhibited variations influenced
by both cultivation duration and Se addition levels．Long-term Se treatment（10 days） inhibited the enrichment
of K，Ca，Mg，and B in shoot．Prolonged exposure to high Se concentrations（10 days）enhanced transport
of K，Mg，Zn，and Mn，while inhibiting B，Cu，and Ni transport，though it did not affect Fe transport．
Total Se accumulation in shoots peaked on day 10，with 20 plants accumulating 6.09 μg（1.0 mg · L-1 Se
treatment）．The 2.0 mg · L-1 Se treatment significantly inhibited total element accumulation on day 8，and
total element accumulation decreased significantly with increasing Se concentration on day 10．In summary，
cultivation for 8 or 10 days with 1.0 mg · L-1 Se represents optimal conditions for producing Se-enriched pea
shoots，yielding higher shoot production，Se enrichment levels，and total element accumulation．This study
provides theoretical guidance and empirical data for the production of Se-enriched pea shoots．
