SUN Xiangyu1, 2, GONG Shiqi1, 2, LUO Yusong1, 2, ZHANG Chengtao1, 2, SONG Huijuan1, 2, SUN Longjun3, HE Changzheng1, 2, TANG Bingqian1, 2, SUN Xiaowu1, 2, DAI Sihui1, 2
Abstract:In this study,employed bioinformatics methods to analyze the physicochemical properties,conserved domains,transmembrane domains,and secondary/tertiary protein structures of the watermelon ERECTA gene(gene ID:Cla97C05G096750).Additionally,phylogenetic tree clustering analysis was performed.The full-length CDS sequence of the watermelon ERECTA gene was cloned,and its tissue-specific expression patterns under abiotic stresses were investigated .The results revealed that the ERECTA gene spans 6 894 bp,containing 27 exons and 26 introns,and is located on chromosome 5(25 867 785-25 874 678 bp),encoding 991 amino acids .Phylogenetic analysis indicated that this amino acid sequence shares a close evolutionary relationship with melon and cucumber,while showing the most distant relationship with rice.qRT-PCR demonstrated that the ERECTA gene exhibited higher expression levels in female fl owers and leaves,but the lowest in stems. Under salt stress,its expression was upregulated,whereas it showed an initial increase followed by a decrease under drought and cold stresses,suggesting its potential role in stress responses. This study provides a foundation for future functional investigations of this gene in watermelon and targeted improvement of key agronomic traits.