（School of Horticulture and Plant Protection，Yangzhou University，Agriculture and Agricultural Product Safety International
Cooperation Joint Laboratory，Yangzhou University，Ministry of Education，Yangzhou 225009，Jiangsu，China）
Abstract：Solanum khasianum C. B. Clarke is an affinis wild eggplant species，possessing valuable
genes resisting both biotic stress and abiotic stress．Taking 10 cultivated eggplants as female parent，
proceeding hybridization with Solanum khasianum C. B. Clarke，and adopting pollination methods as once
pollination，repeating pollination，style cutting back，mixture pollination and pollination after grafting，
etc．，this study had obtained the best way for interspecies hybridizatyon between Solanum melongena L. and Solanum khasianum C. B. Clarke．The results showed that adopting pollination after grafting could increase
hybrid fruit setting rate，and obtain F1 hybrid between L81 and Solanum khasianum C. B. Clarke．SSR
molecular identification was conducted on F1 plant at seedling stage and the F1 seed was proven to be the seed of
interspecific hybridization between Solanum melongena L. and Solanum khasianum C. B. Clarke．
