Development of Inbred Lines of Raphanus sativus L. var.‘Xinlimei’by Isolated#br#
Microspore Culture Technology
ZHANG Li，WANG Qing-biao，WANG Yan-ping
〔Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Key Laboratory of Biology and
Genetic Improvement of Horticultural Crops（North China），Ministry of Agriculture and Rural Affairs，Beijing Key Laboratory
of Vegetable Germplasm Improvement，Beijing 100097，China〕
Abstract：Taking‘Beijing Xinlimei’and‘Shandong Xinlimei’as donor materials，in vitro microspore
culture was carried out．Embryoids were successfully obtained from both genotypes，and the embryo formative
rate of each 105 microspore was 15.6% and 21.3%，respectively．Cytoledon-stage embryoes were chosen to
conduct embryoid regenoration．The planting percents of‘Beijing Xinlimei’and‘Shandong Xinlimei’were
78.9% and 64.5%，respectively．Flow cytometry was used to identify ploidy of regenerated plantlets．44.8%
of them were diploid（double haploid，DH line），17.2% were haploid and 38.1% were polyploid．Normal
DH lines were selected to conduct self-crossing and reserve seed for planting．And field traits identification
were carried out on their progenies．The DH lines exhibited bigger variation compared with the donor plants．
The DH lines derived from‘Beijing Xinlimei’with round fleshy root，green peel，blood red pulp，and oval
green peel，blood red pulp were propagated and their progenies characters were uniform．The rate of blood
red pulp reached 100%，and there were no variant plants with light green pulp．New ‘Xinlimei’lines with
uniformed pulp color were obtained by in vitro microspore culture technology．This had effectively solved the
problem occurred easily during conventional selfing of mutant plants with light green pulp．
