Scab Resistance Evaluation of Potato Advanced Generation and SSR-assisted Screening
SONG Zhi-jun 1，BAI Ya-mei 2，ZHANG Jing-hua 3，ZHANG Shu 3，LI Hui 1，LYU Wen-he 1* ，WEI Qi 3*
1 College of Agriculture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China；2 College of Resources
and Environment，Northeast Agricultural University，Harbin 150030，Heilongjiang，China；3 Potato Research Institute，
Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
Taking 20 potato advanced generation clones created by foreign germplasm resources as test
material，‘Youjin’as susceptible contrast and‘Kexin No.18’ as resistant contrast，this paper evaluated
the scab resistance of test material by natural infested field and artificial inoculation methods；analyzed the
genetic relationship between test materials using SSR molecular markers；and compared the clustering results
of SSR genetic similarity coefficient and classification results of disease resistance of the tested materials，
so as to explore the feasibility of using SSR molecular marker for screening resources with scab resistance．
Two hundred and seventy-eight bacterial strains with Streptomyces characteristics were separated and purified
from soil and tuber，of which 240 from tuber and 38 from soil．One hundred and twenty-seven bacterial
strains with pathogenicity were all identified as Streptomyces scabies．There were significant differences in
disease resistance among the tested materials，which could be divided into 4 types：high resistant，moderate
resistant，moderate susceptible and high susceptible．Correlation between artificial inoculation and resistance
identification in natural disease nurseries was highly significant（R2=0.946 7）．The resistant type and
susceptible type could be roughly distinguished by SSR genetic similarity coefficient clustering，indicating that
scab resistance was related to the genetic relationship．
