Cloning of Heat Shock Protein Related Gene CmHSP70-5 and Expression Analysis under Heat Stress in Cucurbita moschata
HU Yan-ping 1，ZHOU Yang 2，YANG Chun 3，LIAO Dao-long 1，ZHU Bai-bi 1，WANG Min 1，HUANG Wen-feng 1，ZHANG Wen 1，YUN Tian-hai 1*
1 Institute of Vegetables，Hainan Academy of Agricultural Sciences，Hainan Key Laboratory of Vegetable Biology，Hainan
Vegetable Breeding Engineering Technology Research Center，Haikou 571100，Hainan，China；2 College of Horticulture，
Hainan University，Haikou 570228，Hainan，China；3 Hainan Academy of Agricultural Sciences，Haikou 571100，Hainan，
A heat shock protein gene，naming CmHSP70-5，was isolated from pumpkin cDNA by
polymerase chain reaction （PCR） method based on pumpkin genome sequence．Phylogenetic relationship
of HSP70-5 and its homology were analyzed using bioinformation method．The expression quantity of CmHSP70-5 under heat stress was studied by real-time PCR．The results showed that CmHSP70-5 gene was 1
953 bp，and its coding CmHSP70-5 protein had the closest relative with Arabidopsis AtHSP70-5．CmHSP70-5
gene had a high expression level in roots than in stems and leaves under normal conditions．Real-time PCR
results indicated that under heat stress，the expression of CmHSP70-5 gene in roots，stems and leaves were all
up-regulated and could reach the maximum quantity 3 hours after treatment，indicating that the expression of CmHSP70-5 gene was related to heat stress in Cucurbita moschata．
胡艳平，周扬，杨春，廖道龙，朱白婢，王敏，黄文枫，张文，云天海. 南瓜热激蛋白相关基因CmHSP70-5 的克隆和高温胁迫下的表达分析[J]. 中国蔬菜, 2019, 1(8): 28-33.
HU Yan-ping，ZHOU Yang，YANG Chun，LIAO Dao-long，ZHU Bai-bi，WANG Min，HUANG Wen-feng，ZHANG Wen，YUN Tian-hai. Cloning of Heat Shock Protein Related Gene CmHSP70-5 and Expression Analysis under Heat Stress in Cucurbita moschata. China Vegetables, 2019, 1(8): 28-33.