Taking‘ Jiadingbaisuan’ as experiment material，this paper studied the influence of different
detoxication methods of stem tips and different explant initiation medium on the establishment of aseptic seedlings
and virus-free seedling rate. The paper also studied the effects of different propagation medium and cutting
pattern on the initiation and propagation of the potential bud. The paper also studied the grade standard of tissue
culture seedlings and medium component on the formation of tissue culture microbulb. The results showed that
stem tips with a single phyllopodium had the maximal survival rate at 76% in MS+6-BA 1.0 mg·L-1+NAA 0.1
mg·L-1 medium. The single head bud cutting pattern - cutting its stem base at 1 cm by half through axis had the
maximal potential bud propagation coefficient at 3.22 in MS+6-BA 1.0 mg·L-1+NAA 0.10 mg·L-1 medium.
Bud primordium wrapped by one phyllopodium had the best detoxification effect reaching 100%. One single tissue
culture bud with cauloid’s diameter 3 mm had the highest tissue culture microbulb induction rate at 94.8%，in
MS+NAA 0.1 mg·L-1+ Sugar 90 g·L-1 medium with 7.0 pH value. The stem tip detoxification pattern，cutting
pattern of the potential bud，and medium component of tissue culture microbulb were the key technology for
detoxification and rapid propagation of‘ Jiadingbaisuan’.