In order to clear and definite species of squash virus diseases in Beijing area and their main infection pathogens,this paper collected 84 squash samples with suspected virus-like symptoms during 2016-2017 from Beijing area,and carried out detection by RT-PCR used 6 pairs of specific virus primers.The results
showed that 79 samples were positive,among which Cucumber mosaic virus (CMV) had the highest detection rate -52.38%,followed by Zucchini yellow mosaic virus( ZYMV) and Watermelon mosaic virus( WMV).Their detection rate were 44.01% and 14.29%,respectively.Other viruses were not yet detected.In addition,16.67% samples were mix-infected by 2 kinds of viruses.Mix infection of CMV and ZYMV took 7.14%.Mix infection of ZYMV and WMV took 9.52%.CMV was the prevalent pathogenic virus in Beijing area,where the mix infection of viruses on squash was existed.