Studies on Optimization of Cabbage Isolated Microspore Culture Conditions and#br#
Generation of Embryoids from High-generation Inbred Lines
SU He-nan，HAN Feng-qing，YANG Li-mei，ZHUANG Mu，ZHANG Yang-yong，WANG Yong，LI Zhan-sheng，FANG Zhi-yuan*，LYU Hong-hao*
Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Key Laboratory of Biology and Genetic
Improvement of Horticultural Crops，Ministry of Agriculture，Beijing 100081，China
Taking 25 different types of cabbage（Brassica oleracea L. var. capitata L.）accessions as
material，this paper studied on the factors affecting the generation of embryoids in culturing isolated microspore
and conducted optimization．The embryogenesis for high-generation inbred lines was studied as well.The
results showed that the donor plant genotype is the key factor affecting embryogenesis．Embryoids were induced
from 11 accessions among all the tested materials．The highest embryoid inducing rate was 19.8 per bud in the
hybrid‘Zhonggan 628’and 47.5 per bud in inbred line‘01-88’，which were extremely significantly higher
than that of the other accessions．Floral buds with anther length between 3.0-3.5 mm，and ratio of anther length to
petal length of 3∶2-2∶1，could produce the highest proportion of microspores at late-uninucleate stage．Early
and florescence stages were the optimum sampling periods．Adding active carbon could extremely significantly
improve embryoid induction rate．
