Establishment of a Regeneration System in Wax Gourd（ Benincasa hispida Cogn.）
LI Zhifang1，YAN Jinqiang2，HAN Xiangfeng1，XIE Dasen2，LIU Wenrui2，WU Qianhui1，JIANG Biao2*
1Foshan Institute of Agricultural Sciences，Foshan 528145，Guangdong，China；2Vegetable Research Institute，Guangdong
Academy of Agricultural Sciences，Guangzhou 510640，Guangdong，China
Abstract：In this study，taking cotyledon nodes as explants，the effects of different proportions and
concentrations of hormones and days of dark culture on cotyledon node callus-induced regeneration buds were
optimized，the effect of different concentrations of hormone combinations on adventitious bud proliferation
were studied，and the rooting and transplanting of regenerated seedlings were tested，finally we established
a relatively comprehensive set of regeneration system of wax gourd cotyledon nodes．According to the result
of orthogonal experiment，the optimum combination for inducing adventitious bud differentiation was MS +
2，4-D 0.1 mg · L-1 + IBA 0.10 mg · L-1 + 6-BA 0.8 mg · L-1 after 7 days dark culture；and the time of dark
culture and concentration of 2，4-D were major factors affecting regeneration．The best medium for proliferation
of adventitious bud was MS + NAA 0.05 mg · L-1 + 6-BA 0.2 mg · L-1，with a proliferation coefficient of 2.487．
The rooting rate of the regenerated seedlings on the rooting medium was 100% on 1/2MS + NAA 0.2 mg · L-1 and the survival rate of the domesticated seedlings was more than 80% after transplanting．
李志芳，闫晋强，韩向峰，谢大森，刘文睿，伍倩慧，江彪. 冬瓜离体再生体系的建立[J]. 中国蔬菜, 2021, 1(4): 59-63.
LI Zhifang，YAN Jinqiang，HAN Xiangfeng，XIE Dasen，LIU Wenrui，WU Qianhui，JIANG Biao. Establishment of a Regeneration System in Wax Gourd（ Benincasa hispida Cogn.）. China Vegetables, 2021, 1(4): 59-63.
