Establishment of a Regeneration System in Wax Gourd( Benincasa hispida Cogn.)
LI Zhifang1,YAN Jinqiang2,HAN Xiangfeng1,XIE Dasen2,LIU Wenrui2,WU Qianhui1,JIANG Biao2*
1Foshan Institute of Agricultural Sciences,Foshan 528145,Guangdong,China;2Vegetable Research Institute,Guangdong
Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China
Abstract:In this study,taking cotyledon nodes as explants,the effects of different proportions and
concentrations of hormones and days of dark culture on cotyledon node callus-induced regeneration buds were
optimized,the effect of different concentrations of hormone combinations on adventitious bud proliferation
were studied,and the rooting and transplanting of regenerated seedlings were tested,finally we established
a relatively comprehensive set of regeneration system of wax gourd cotyledon nodes.According to the result
of orthogonal experiment,the optimum combination for inducing adventitious bud differentiation was MS +
2,4-D 0.1 mg · L-1 + IBA 0.10 mg · L-1 + 6-BA 0.8 mg · L-1 after 7 days dark culture;and the time of dark
culture and concentration of 2,4-D were major factors affecting regeneration.The best medium for proliferation
of adventitious bud was MS + NAA 0.05 mg · L-1 + 6-BA 0.2 mg · L-1,with a proliferation coefficient of 2.487.
The rooting rate of the regenerated seedlings on the rooting medium was 100% on 1/2MS + NAA 0.2 mg · L-1 and the survival rate of the domesticated seedlings was more than 80% after transplanting.
李志芳,闫晋强,韩向峰,谢大森,刘文睿,伍倩慧,江彪. 冬瓜离体再生体系的建立[J]. 中国蔬菜, 2021, 1(4): 59-63.
LI Zhifang,YAN Jinqiang,HAN Xiangfeng,XIE Dasen,LIU Wenrui,WU Qianhui,JIANG Biao. Establishment of a Regeneration System in Wax Gourd( Benincasa hispida Cogn.). China Vegetables, 2021, 1(4): 59-63.