Molecular Marker Assisted Selective Breeding of Radish Male Sterile Line
ZHANG Li，WANG Qing-biao，WANG Yan-ping
〔Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Key Laboratory of Biology and
Genetic Improvement of Horticultural Crops （North China），Ministry of Agriculture and Rural Affairs，Beijing Key Laboratory
of Vegetable Germplasm Improvement，Beijing 100097，China〕
Abstract：The fertility genotypes in 6 fruit radish varieties during seedling stage were identified by specific
marker of Ogura CMS fruitless gene orf138 and KASP marker related to nuclear fertility restore gene Rfo．
The result showed that the cytoplasm of these 6 varieties were normal and there were differences existing in
genotypes of nuclear fertility restore gene Rfo among different single plant．Among 60 identified single plant，
the genotype rfrf，Rfrf，RfRf accounted for 40.0%，33.3% and 26.7%，respectively．Taking while radish
cultivar from Japan‘Kenka 37’as male sterile，this study selected single plant of rfrf genotype to conduct
hybridization and backcross．Four male sterile lines and maintainers of green peel，green pulp fruit radish
with genetic stability，high uniformity and 100% sterile rate were obtained by 5 generations backcross and selfcrossing．
Seven high quality hybridized combination were obtained using these 4 male sterile lines．Applying
molecule marker assisted selection in developing radish male sterile lines could avoid compounding vast cross
combination and fertility identification in the fields，and also improve the selecting efficiency and accuracy．
