Expression of SlTCPs Transcription Factors involved in Tomato Leaf Shape#br# Development and Bioinformatic Analysis
ZHANG Xueying，YIN Yige，JIANG Jing，LIU Xin*
College of Horticulture，Shenyang Agricultural University，Key Laboratory of Protected Horticulture，Ministry of
Education，Key Laboratory of Protected Horticulture of Liaoning Province，Shenyang 110161，Liaoning，China
Abstract：TCP is a class of transcription factors unique to plants and plays an important role in plant
growth and development．Selecting 18 TCP members highly expressed in tomato leaf tissue，this study detected
their expression levels at different development stages；and screened out 10 TCP family members related to
leaf blades development process，including SlTCP2，SlTCP4，SlTCP8，SlTCP11，SlTCP14，SlTCP15，
SlTCP19，SlTCP22，SlTCP24，SlTCP25，etc.．Then bioinformatic analysis was performed on them．The
results indicated that these 10 SlTCPs members were distributed in 8 different chromosomal locations．There
were relatively larger differences in their length of basic group，amino acid and molecular weight．And all
of the TCPs were unstable hydrophilic proteins．Analysis of SITCPs protein structure and element indicated
that in center about 50aa length amino acid sequences were relatively well conserved．Moreover，serine took
relatively larger proportion．The site prediction of SlTCPs protein subcellular fraction were all localized on the
nucleus．The above mentioned SlTCPs protein all contained the conserved domains of typical TCP family．
Its classification of conserved motif was consistent with the branching of phylogenetic tree．Results of tissue
expression analysis showed that SlTCP11，SlTCP15 and SlTCP24 all had relatively higher expression levels
in different tissues and organs at each stage．Using typical tomato leaf shape mutant materials LA2922 and
LA2532 and their corresponding wild types，this experiment conducted qRT-PCR．The results indicated that
the expression levels of SlTCP2，SlTCP11，SlTCP14，SlTCP15，SlTCP19 and SlTCP24 in leaf blades of
LA2922 and LA2532 were in the opposit direction．Combining with the results of bioinformation analysis，it
was specified that SlTCP2，SlTCP11，SlTCP14，SlTCP15，SlTCP19，and SlTCP24 might play a key role in
the development of tomato leaf shape．This has laid foundation for further explorating the molecular mechanism
of tomato leaf blades growth and development．
