CHANG Jing，WANG Zemin，HU Yunjie，HE Wenjing，JIN Xin，HOU Lele，LI Xuan，AI Qianyi
To explore the role of the StCDFs transcription factor family in potato stress response，bioinformatics methods were employed to analyze the phylogenetic relationship，gene structure，conserved motifs，cis-acting elements，tissue-specific expression，hormone and stress response of StCDFs．Simultaneously，transcriptome data and qRT-PCR were used to analyze the transcription levels of StCDFs after diff erent cold-tolerant varieties were treated with low temperature stress．A total of 5 StCDFs members（StCDF1，StCDF2，StCDF3，StCDF4，StCDF5）were identified in potato．The CDF family of potato is closely related to tomato．The analysis of gene structure and conserved Motif revealed that Motif 1 was the most widely distributed and conserved in the potato StCDF．Cis-acting element analysis showed that the upstream promoter of StCDFs gene contained a wide range of hormone and stress response elements，and stress response elements accounted for the largest proportion．Tissue-specifi c expression analysis revealed that StCDFs exhibited distinct expression patterns in diff erent tissues．Additionally，most of these genes were found to respond to stress treatment．Based on the RNA-seq data，the StCDFs were found to respond positively to low temperature stress treatment．Subsequently，the qRT-PCR was performed to explore the expression levels of StCDFs and found that the expression levels of StCDF1，StCDF2 and StCDF3 were up-regulated under low temperature stress．
