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01 October 2020, Volume 1 Issue 10
    

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  • Research Progress on Salt Tolerance in Cucurbita
    WANG Wei-qi1，ZHANG Meng2，QIN Zhao-chen1，MA Wei2，SUN Ting-zhen2，SHI Yu-zi2，DUAN Ying2*，WANG Chang-lin2*
    China Vegetables. 2020, 1(10): 18-26.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Abstract：Soil salinization is one of the main abiotic stresses in vegetable production．Pumpkin
    （Cucurbita spp.）displayed strong tolerance to salt stress and was used as rootstock in grafting cultivation of
    cucumber，melon and watermelon．Selection of pumpkin varieties with salt tolerance is helpful to improve
    the yield and economic benefits of pumpkin and Cucurbitaceae grafted vegetables．This paper summarized the
    research progress on evaluation methods for salt tolerance of Cucurbita and salt-tolerant Cucurbita germplasm
    screening，physiological mechanism in forming Cucurbita salt tolerance，inheritance of Cucurbita salt
    tolerance traits and related salt tolerance genes in Cucurbita．Furthermore，the paper also prospected the future
    research direction，hoping to provide more theoretical references for understanding the regulatory mechanism
    of pumpkin salt tolerance，and selection of salt tolerant varieties．
  • Comparative Analysis and Application of Rapid Detection on Potato Soil-borne Pathogens
    LI Lei，ZHAO Yu-rong，XIE Xue-wen，SHI Yan-xia，CHAI A-li，LI Bao-ju*
    China Vegetables. 2020, 1(10): 27-34.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Potato soil-borne diseases are becoming increasingly serious．Rapid detection on pathogenic
    bacteria is an important basis for early diagnosis，predictive monitoring，and comprehensive control of the
    diseases．This paper comprehensively compared the characteristics of conventional PCR，nested PCR，realtime
    quantitative PCR，multiplex PCR and LAMP technology in sensitivity and specificity，and evaluated their
    merits and demerits in detecting potato soil-borne diseases．The paper also summarized the primer information
    of various diseases detection reports and advantages of this detection system．At the same time，the paper
    prospected the applying future of each technology，aiming at obtaining an overall understanding about the rapid
    detection methods and their development tendency on potato soil-borne diseases．
  • Analysis on Cloning and Expression of ACC Synthase（CqACS）Gene from Benincasa hispida
    PENG Qin2，WANG Min1，3，PENG Qing-wu1，JIANG Biao1，3，LIN Yu-e1，LIU Wen-rui1，3，LIANG Zhao-jun1，LI Ling2，HE Xiao-ming1，3
    China Vegetables. 2020, 1(10): 35-41.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to find out the relation between ACC synthase and female chieh-qua，this study took
    female inbred line（A36）and normal monoacious inbred line（SX）as material，gained CqACS gene through
    coloning，then conducted homologous sequence comparative analysis with the known CqACS gene；and
    constructed evolutionary tree．The expression profile of CqACS in seedling leaf blades of A36 was analyzed
    after treatment with gibberellin（GA3）by qRT-PCR on CqACS gene．The female flower rate within 20 nodes
    was carried out statistic analysis before and after gibberellin treatment．The results showed that the length of
    CqACS gene in A36 of female inbred line was 1 318 bp and that in normal monoecism selfing line in SX was
    1 637 bp．After comparative analysis，we found that SX in 3 bp base element：91，97 bp and 117 bp were
    more than detected in A36，and only 2 basic group differences were found between them．The homologous
    sequence comparison revealed that CqACS gene in A36 had the highest homology with Cit-ACS1 gene in
    watermelon，reaching 95.31%．And the homology with cucumber Cs-ACS1 was 91.31%．CqACS gene in SX
    had the homology with Cit-ACS1 gene in watermelon，reaching 78.04%，and the homology with cucumber
    Cs-ACS1 was 76.06%．After GA3 treatment，the average female flower rate（within 20th node order）of GA3
    treatment group in A36 was 45%．While，that of the contrast group was 90%．qRT-PCR analysis discovered
    that the expression quantity of CqACS gene after GA3 treatment was significantly up-regulated．Compared with
    the contrast group．Moreover，4 h after the third time treatment the up-regulated range was the largest．
  • Genetic Diversity Analysis of Eggplant Germplasm Resources Based on InDel Markers
    ZHANG Qiang-qiang1，3，JIANG Hai-kun1，2，WANG Yan1，2，LIANG Sai1，4，SUI Yi-hu3*，JIA Li1，2*，FANG Ling1，2，ZHANG Qi-an1，2，DONG Yan-xiang1，2
    China Vegetables. 2020, 1(10): 42-47.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In this experiment，19 pairs of InDel markers were used to study the genetic diversity of 46
    germplasm resources of eggplant from home and abroad．The results showed that the PIC values of 19 pairs
    of InDel markers ranged from 0.48-0.66，with 0.59 mean value．The genetic similarity coefficient of 46
    eggplant germplasm resources was between 0.32-1.00 with 0.70 mean value，indicating little genetic difference
    between 46 eggplant germplasm resources and with relatively narrow genetic basis．UPGMA method was
    used for clustering analysis，and 46 germplasm resources of eggplant were divided into 4 groups at the genetic
    similarity coefficient of 0.66．The clustering result had certain relevance with leaf color，corolla color and fruit
    characters，while had little relevance with area of source．
  • Changes of Endogenous Hormone Content in Watermelon Varieties with Different Branching Forms During Their Growth Process
    YANG Yong-gang，ZHANG Hua-sheng，LI Xiao-fang，SU Yong-quan
    China Vegetables. 2020, 1(10): 48-54.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
     Taking multi-branch watermelon variety ‘Longkang No.9’ and no branch watermelon variety
    ‘Wuchazao’ as experimental materials，this paper studied and analyzed the changes of endogenous hormone
    content，including Indole-2-carboxylic acid（ICA），indole-3-acetic acid（IAA），zeatin（Z），dihydrozeatin
    （DHZ），abscisic acid（ABA），gibberellin（GA1，GA3，GA4 and GA7） by HPLC-MS/MS during
    watermelon growth period．The results showed that the contents of ICA，ICA+IAA in watermelon leaves of no
    branch variety were significantly higher than that of multi-branch variety during vine spreading period and fruitexpanding
    early stage．During the whole growth period，the contents of Z，DHZ+Z in no branch variety were
    lower than that of multi-branch variety．Furthermore，the ratios of （ICA+IAA）/（Z+DHZ），（ICA+IAA）/
    ABA，（ICA+IAA）/（GA1+GA3+GA4+GA7） in no branch variety were significantly higher than that of multibranch
    variety during vine spreading period and early stage of fruit expanding．Thus，it could be seen that
    higher contents of ICA could inhibit the branching of watermelon side shoots；while the higher content of DHZ
    and Z could promote the form of watermelon lateral branches．The higher ratios of（ ICA+IAA）/（Z+DHZ），
    （ICA+IAA）/ABA，（ICA+IAA）/（GA1+GA3+GA4+GA7） were harmful to the formation of water melon
    branches．
  • Comparison and Physiological Traits of Main Agronomic Characters of Nuclear#br# Sterile Lines in Pepper
    LIANG Sai1，2，JIA Li1，3，WANG Yan1，3，ZHANG Qiang-qiang1，3，CHEN You-gen2*，JIANG Haikun1，3*，FANG Ling1，3，ZHANG Qi-an1，DONG Yan-xiang1，3
    China Vegetables. 2020, 1(10): 55-61.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Taking 2 pepper nuclear male sterile lines‘GMSDS-702A’and‘GMSDS-702B’as
    experimental material，this paper conducted investigation on their main agronomic traits，and measured the
    contents of relevant physiological indicators at different development stages of flower buds．The results showed
    that the vegetative growth of sterile line plant was relatively vigorous．The plant height and spread width were
    significantly higher than that of the fertile line．The anthers of the sterile line were shriveled，with browned
    surface，the tissue structure was hardened，and the anthers were tightly closed and could not form normal
    pollen grains．The contents of soluble sugar，soluble protein，free proline，and malondialdehyde were all
    different with various degrees at different development stages．At the stage of microspore mother cell，the
    contents of soluble sugar and free proline in sterile lines were reduced by a big margin，and the content of
    malondialdehyde was at a higher level．Those of，fertile lines were on the contrary．It might have a certain
    inner link with the abortion of microspore．
  • Obtain and Identification of F1 hybrid of Solanum melongena L. and Solanum khasianum C. B. Clarke
    XUE Jin-yan，LIU Yi-yong，WU Jun-xiang，XU Zhao-can，ZHANG Yu，CHENG Yu-fu，YANG Xu
    China Vegetables. 2020, 1(10): 62-67.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Solanum khasianum C. B. Clarke is an affinis wild eggplant species，possessing valuable
    genes resisting both biotic stress and abiotic stress．Taking 10 cultivated eggplants as female parent，
    proceeding hybridization with Solanum khasianum C. B. Clarke，and adopting pollination methods as once
    pollination，repeating pollination，style cutting back，mixture pollination and pollination after grafting，
    etc．，this study had obtained the best way for interspecies hybridizatyon between Solanum melongena L. and
    Solanum khasianum C. B. Clarke．The results showed that adopting pollination after grafting could increase
    hybrid fruit setting rate，and obtain F1 hybrid between L81 and Solanum khasianum C. B. Clarke．SSR
    molecular identification was conducted on F1 plant at seedling stage and the F1 seed was proven to be the seed of
    interspecific hybridization between Solanum melongena L. and Solanum khasianum C. B. Clarke．
  • Identification of Hyacinth Bean Sclerotinia Pathogen and Its Biological Characteristics
    GU Yu-yang1，WANG Li-na2，YUAN Juan3，ZHANG Yi1，YAO Lu-ming1，MA Xiao-hong1，WANG Biao1*，WU Tian-long1
    China Vegetables. 2020, 1(10): 68-76.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In recent years，a sclerotinia disease similar to symptoms of the other crops has broken out
    at the main hyacinth bean producing area in Shanghai Pudong district．Through conducting mophological
    observation，pathogenicity identification on separated and purified pathogenic bacteria，analyzing molecular
    biology characteristics，we confirmed that the pathogen causing this disease was Sclerotinia sclerotiorum
    （Lib．）de Bary．This pathogen could infect the whole plant of hyacinth bean，including its root，stem，
    leaf，flower and pod．We had also observed the formation of its sclerotia．This study discovered that the
    best growth carbon source for indoor seperation and culture of Sclerotinia sclerotiorum was fructose at 22 ℃，
    pH 5．Proper growth conditions under stress were in favour of forming sclerotia．There were differences
    existed in ITS rDNA sequences of Sclerotinia sclerotiorum from hyacinth bean，soybean and pakchoi．
    Comparing Sclerotinia sclerotiorum originating from those different sources，we found that those strains
    belonged to different fusion groups，and they had discrepant pathogenicities to different hosts，but all of them
    could cause diseases．
  • Analysis on Quantity，Distribution Characteristics and Utilization as Resource Manure Potential of Different Kinds Vegetable Wastes in Hebei Province
    DUAN Ya-jun1，ZHANG Hui1，LYU Shi1，ZHANG Yu-kun1，WANG Xiao-min1，NING Guo-hui1，YANG Zhi-xin1，2*
    China Vegetables. 2020, 1(10): 77-82.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Taking 8 kinds of main vegetable wastes in 11 prefecture-level cities of Hebei Province as study
    object，the paper estimated the quantity of different vegetable wastes resources in Hebei Province by grassgrain
    ratio．The results showed that the quantity of vegetable waste resources in Hebei Province increased at
    an average rate of 42 600 t · a-1 from 2011-2016，reaching 2.30 million t in 2016．Among which，cabbage
    （578 700 t），Chinese cabbage（434 200 t） and solanaceous vegetables（299 800 t） had the highest quantity
    of vegetable waste resources，increasing by 100.98%，50.80% and 4.12%，respectively compared with
    the mean value，followed by tubers，beans and melon vegetables．Those of leaf and bulb vegetables were
    the lowest．The quantity of waste resources was distributed unevenly among each prefecture-level city，
    with 50.57%-82.52% coefficient variation．Tangshan（408 100 t），Shijiazhuang（351 700 t），Baoding
    （297 800 t） and Zhangjiakou（279 600 t） had the maximum quantities，accounting for 58.04% of the whole
    province．Utilization as resource manure of all these vegetable wastes in the province was equivalent to indirect
    application of 45.15 kg · hm-2 pure nitrogen，27.75 kg · hm-2 pure phosphorus，44.85 kg · hm-2 pure potassium
    and 1 089.15 kg · hm-2 of organic matter per 667 m2 farmland；and equivalent to 6.68% of the annual amount
    of chemical fertilizer and 2.992 million t of organic fertilizer，among which cabbage，Chinese cabbage and
    solanaceous vegetables had the highest nitrogen，phosphorus and potassium nutrients，accounting for 57.73%
    of the 8 kinds vegetables．The organic matter content in tubers vegetables was the highest．The quantity of
    vegetable waste resources in the whole province showed a steady upward trend from 2011-2016．There were
    significant differences in the quantities of all kinds of vegetable wastes and nutrients in different regions，among
    which vegetable yield and waste coefficient had become important factors affecting the difference of vegetable
    waste resources quantities．
  • A New Hot Pepper F1 Hybrid of Mid-maturity —‘Ruizhi’
    WANG Chao-lian，DING Hai-feng，HE Wei-ming
    China Vegetables. 2020, 1(10): 83-85.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    ‘Ruizhi’is a new tapered pepper F1 hybrid developed by crossing thick cow-horn pepper stable
    inbred line‘11-22’as female parent and pimento stable inbred line‘11-431’as male parent．It is of midmaturity．
    The initial flower mostly sets on the 9th node．The plant has strong growth vigor．Its continuous
    fruit setting ability is good．The fruit is in nice order．The upper fruits and down fruits have good consistency．
    The fruit is about 17 cm in length，5 cm in shoulder width and 0.45 cm in pulp thickness with 2 or 3 lobes．
    The single fruit weight is about 130 g．Its fruit has smooth surface with shining glossiness．The young fruit
    is dark green in color．The matured one is dark red in color and changes color quickly with good hardness．
    It tastes very hot．Its resistance to virus disease，bacterial wilt and stem rot in fields is stronger than that of the
    contrast‘Tianza No.1’．At earlier stage，it can yield about 37.5 t · hm-2，and the total yield is about 63 t ·
    hm-2．It is suitable for planting in protected and open fields in Yunnan and Sichuan Provinces．
  • A New Melon F1 Hybrid —‘Chujin No.1’
    WANG Yun-qiang，DAI Zhao-yi，YI Li-cong，GONG Yu，WANG Shu-jing
    China Vegetables. 2020, 1(10): 86-88.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    ‘Chujin No.1’is a new melon F1 hybrid of medium and late maturity developed by crossing
    inbred line‘TH607’as female parent and inbred line‘TH605’as male parent．Its whole growth period is
    about 103 days，and the fruit development period is 42-45 days．The plant growth vigor is strong．Its branch
    ability is medium．The leaf is green in color．The fruits mainly bear on lateral branches．The fruit is of round
    shape and pericarp is yellow with dark green plaques．The average single fruit weight is about 1.5 kg．Its flesh
    is white in color with about 3.7 cm thickness and soft quality．The content of center soluble solid is about 16%．
    The fruit quality is fine with good taste．In fields，its resistance to powdery mildew and downy mildew was
    amount to that of the contrast‘Yilishabai’．Its yield under plastic greenhouse by vine hanging cultivation was
    about 37.5 t · hm-2．It is suitable for facility cultivation in spring and autumn at the middle and lower reaches of
    the Yangtze River．
  • A New Tomato F1 Hybrid with Resistant to TYLCV —‘Longfan No.1’
    HOU Fu-en，HAO Ke-xing*，SU Dong-tao，ZHANG Tao，WANG Ming，ZHANG Man，HOU Dong-ying
    China Vegetables. 2020, 1(10): 89-92.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Abstract：‘Longfan No.1’is a new tomato F1 hybrid developed by crossing inbred line‘TMX2511-2’as
    female parent and‘TMX2535-4’as male parent．It is of indeterminate growth type．The plant has strong
    growth vigor．The first female flower node order was at 8-9th node．The fruit is red in color and of oblate
    shape with 6.5-7.0 cm vertical diameter，and 8.0-8.5 cm transverse diameter．The average single fruit weight
    is about 220 g．The fruit is of medium firmness．Its young fruit has no green shoulder．The fruit soluble solid
    content is 5.9%，lycopene content is 0.035 mg · g-1，VC content is 0.167 mg · g-1．It can generally yield about
    127.5 t · hm-2．It is highly resistant to TYLCV，resistant to leaf mold，and moderate resistant to CMV and root
    knot nematode．This variety is suitable for planting under plastic greenhouse in early spring and late autumn at
    Lyuliang，Xinzhou，Jinzhong，Datong，Changzhi of Shanxi Province．
  • New Erythropalum scandens Blume Varieties with Medicinal and Edible Vegetable —‘Guichicangteng No.1’and‘Guichicangteng No.2’
    ZHANG Shang-wen，LI Ting，SHI Qian，PAN Ying-nan，YU Wei-hua，MENG Ping，ZHANG Xiang-jun*
    China Vegetables. 2020, 1(10): 92-95.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Abstract：‘Guichicangteng No.1’and‘Guichicangteng No.2’are excellent strains of new medicinal and
    edible vegetable varieties screened from the wild resources in Guangxi and bred mainly by rapid propagation via
    tissue culture，supplemented by cutting propagation．The tender shoots of‘Guichicangteng No.1’are green
    in color，with a yield of 28.5 t · hm-2．The tender shoots of‘Guichicangteng No.2’are red in color．Its flavor
    is more rich than that of‘Guichicangteng No.1’．‘Guichicangteng No.2’can yield 25.5 t · hm-2．These 2
    varieties all have axillary tendril and slender branches，on which there are indistinct stripes．The leaves change
    from papery to thick papery or subleathery and were 8-20 cm in length，4-15 cm in width．The fresh tender
    buds are about 15 cm in length with special flavor．The contents of protein，fat，coarse fiber，calcium，
    phosphorus，VC and total amino acid content are higher than that of normal vegetables．Three years after field
    planting，these 2 varieties will enter into a high-yielding period．They are suitable for open fields cultivation in
    Guangxi and Southwestern areas，and protected cultivation in northern regions．
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