GUO Yu,GUO Hai-yan,LUO Lan,LIU Shi,GAO Ye,ZHANG Tai-feng,LUAN Fei-shi,WANG Xue-zheng
China Vegetables. 2020, 1(5): 26-33.
In this experiment,200 F2 populations were obtained from green stigma melon ‘MR-1’and yellow stigma melon ‘TN’ as parents,and based on resequencing the SNP-CAPS markers and BSA technology were developed for construction of the molecular map.A genetic linkage map containing 12 linkage groups and 135 CAPS markers was constructed,covering a genome length of 2 731.97 cM.QTL analysis was conducted on the related characters of melon stigma color(yellow or green) by this map.Twelve QTLs related to stigma color were obtained.There were 4 QTLs related to stigma total chlorophyll content,3 QTLs related to stigma carotenoid content,2 QTLs related to stigma color card,and 3 QTLs related to the ratio of stigma total chlorophyll content to stigma carotenoid content,distributing in 1,2,4,5,7,8,11,12 on the linkage group,explaining 4.63%-20.44% phenotypic variation.The main effect sites regulating stigma total chlorophyll content,stigma carotenoid content and stigma color card were SChC-1-1,SCaC-12-1and SCoC-2-1.Their contribution rates were 18.78%,12.08%,20.44%,respectively.