China Vegetables 2016 Vol.1

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China Vegetables    2016, 1 (1): 1-7.  
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China Vegetables    2016, 1 (1): 8-12.  
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China Vegetables    2016, 1 (1): 12-16.  
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China Vegetables    2016, 1 (1): 17-17.  
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Research Progress in Tomato Ascorbic Acid Biosynthesis and Metabolism
ZHANG Feng-xia, TAO Pei-wen, LI Han-xia, YE Zhi-biao, ZHANG Yu-yang*
China Vegetables    2016, 1 (1): 18-23.  
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Ascorbic acid is an important antioxidant synthesized in plant. It has an important role in plant growth and development. Biosynthesis and metabolism of ascorbic acid is summarized and its metabolism regulation in tomato is emphasized in this paper. The future research direction about ascorbic acid metabolism is also prospected.

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Development and Application of dCAPS and InDel Markers in Pink Tomato Fruitrelated Genes
DONG Shu-fang,WANG Xiao-xuan,GAO Jian-chang,GUO Yan-mei,HUANG Ze-jun,DU Yong-chen*
China Vegetables    2016, 1 (1): 24-29.  
Abstract902)      PDF(pc) (1386KB)(1867)       Save

Super BSA was performed to screen SNP between 2 mixed DNA pools,which contained 100 pink tomatoes and 100 red tomatoes.SNPs of the tested result were analyzed.The result indicated that 6 SNPs among 127 SNPs on chromosome 1 were highly correlated with the color of tomato fruit,and 1 SNP was closely linked to pink tomato fruit.By this SNP,a polymorphism dCAPS was developed and showed well correlation with red,pink and heterozygous genotypes.It is recently reported that a 603 bp deletion was characterized at the upstream of SIMYB12 gene in pink tomato.Using this deletion,a InDel marker was developed and also showed good polymorphism among red,pink and heterozygous genotypes.These 2 newly developed molecular markers
were further used to analyze genetically the F2 population.The result was in line with the field observation.Their accordance rate was above 95%,which suggested that these 2 markers can be used for molecular marker assisted selection in tomato breeding.

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Studies on Origin and Evolution of Tuber Mustard(Brassica juncea var. tumida Tsen et Lee)based on Chs Gene Sequences
CHEN Fa-bo,YAO Qi-lun*,Dong Er-fei,FANG Ping
China Vegetables    2016, 1 (1): 30-36.  
Abstract429)      PDF(pc) (2036KB)(1269)       Save

In order to explore the origin and evolution of tuber mustard(Brassica juncea var. tumida Tsen et Lee),we cloned and sequenced the Chs gene sequences of tuber mustard and its close relative species.As illustrated in the phylogenetic tree,the sequences from all Brassica taxa were separated into three well supported clades and nine sub-clades.Splits tree analysis indicated that there existed not only tree evolution,but also reticulate evolution among tuber mustard and its related plants.Median-joining(MJ)network analysis showed that B. rapa and B. nigra were the parental donors of A genome and B genome in allotetraploid B. juncea tumida,respectively.Tuber mustard,as a variety of B. juncea,was closely related to B. juncea var. utilis,indicating that B. juncea tumida might originate from B. juncea var. utilis.In addition,the single copy gene Chs could be efficiently applied to evaluate the phylogenetic relationships of tuber mustard its and related plants.

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Evaluation of Melon Germplasm Resistant to Fusarium Wilt and Powdery Mildew in Seedling Stage
NAN Yu-hang,ZHU Zi-cheng,WANG Xue-zheng,LI Liang-rui,LUAN Fei-shi*
China Vegetables    2016, 1 (1): 37-44.  
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The resistances to Fusarium oxysporum f. sp. melonis race1 and Sphaerotheca fuliginea race 1 of 126 melon germplasm resources from China and abroad were identified by single and composite inoculations with spore suspension in seedling stage,respectively. The result indicated that under single inoculation condition,7 were highly resistant to Fusarium wilt,42 were resistant,61 were mid-resistant,15 were susceptible and 1 was highly susceptible.While 9 were highly resistant to powdery mildew,42 were resistant,72 were mid-resistant,and 3 were susceptible. Under composite inoculation condition,PI164331 introduced from Indian was screened as resistant to both Fusarium wilt and powdery mildew. Significant positive correlations were observed between the evaluation results of single inoculation and composite inoculation,though correlation analysis of these 2 methods.

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Effect of Polyolefin Film on Thermal and Light Condition and Yield of Cucumber in Solar Greenhouse
LI Yan-su1,WANG Hui-jun2,YAN Yan1,HE Chao-xing1,YU Xian-chang1*,MI Qing-hua3*
China Vegetables    2016, 1 (1): 45-48.  
Abstract444)      PDF(pc) (1457KB)(1273)       Save

In order to evaluate the application value of polyolefin(PO)film for cucumber growth in solar greenhouse,a greenhouse coated with ethylene-vinyl acetate(EVA)film was served as the contrast.The influence of PO film coating on temperature and illumination environment and yield of cucumber(Cucumis sativus L.)in solar greenhouse were studied.The results showed that the light intensity in PO solar greenhouse was increased about 20-280μmol·m-2·s-1 .As a result,the daily average air and soil temperature were increased 0.2-2.0 ℃,0.3-1.5 ℃,respectively compared with the EVA solar greenhouse.There were no significant effects on carpopodium length and fruit diameter.However,the fruit length were increased significantly by PO film coating.The commercial rate of cucumber fruit were increased about 10 percentage points,which result in the increase of cucumber yield.Compared with the yield of cucumber in EVA solar greenhouse,the earlier,middle and later yield in PO solar greenhouse were increased about 52.0%,27.2% and 58.6%,respectively.So the acre yield and income were increased about 43.4% and 39.9%,respectively,and the input-output ratio were increased
about 129%.PO film had a good application prospective in protected vegetable production in northern China in winter and spring.

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Cytomorphological Observation on Three Kinds of Sexual Type Sex Differentiation in Watermelon
SHAN Wen-ying,JI Gao-jie,ZHANG Jie,GONG Guo-yi,ZHAO Hong,XU Yong,ZHANG Hai-ying*
China Vegetables    2016, 1 (1): 49-54.  
Abstract356)      PDF(pc) (3091KB)(1598)       Save

 Taking male,female and hermaphrodite flower of 3 kinds of watermelon(Citrullus lanatus)sexual type as material, we made these flower buds at different development stages into paraffin section for cytomorphological observation.The result showed that the floral primordial processes of these 3 kinds of sexual type from watermelon had no visible differences. At the end of hermaphroditic stage,the length of flower bud was about 0.8-1.0 mm and the differentiation development of male,female and hermaphrodite flower began. Stamen primordia volume of male and hermaphrodite flower augmented rapidly,and carpel primordia of female and hermaphrodite flower began to differentiate into stigma and ovary.In deformity female flower,the base of carpel primordia would stop the development after denting to a certain degree,while the stamen primordia would not stop elongation and continue to develop to form the mature stamen with small amount of mature pollen grains.

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Heavy Metal Enrichment and Population Health Risk Assessment of  Vegetables in Baotou Industrial District
LI Li-man1,LIU Ju-mei1,SHEN Wei-shou2,JIANG Hai-ming1,BIAN Xiao-yan1,LIANG Dong1,SI Wan-tong1, 2*
China Vegetables    2016, 1 (1): 54-59.  
Abstract382)      PDF(pc) (1485KB)(1088)       Save

The vegetable and soil were sampled in Baotou Nanhaizi Industrial District,and the heavy metals contents of all samples were determined with the inductively coupled plasma mass spectrometry(ICP-MS) .The heavy metals were Hg,Se,Zn,Cu,Pb,Mn,Cd,and the vegetables were Apium graveolens L.,Lactuca sativa L., Spinacia oleracea L., Coriandrum sativum L., Lactuca sativa L. var. longifolia Lam.,Brassica campestris L. ssp. pekinensis(Lour)Olsson, Brassica campestris L. ssp. chinensis(L. )Makino var. communis Tsen et Lee.The soil pollution levels and the local resident health risk from vegetables intake was also assessed. The results showed that soil in Baotou Nanhaizi Industrial District was moderately polluted by heavy metals,among which Hg and Se pollution were very serious.The migration abilities to vegetables of different heavy metals were different, among them,those of Mn,Cu and Zn were bigger in all vegetables,and their risk to residents’health was also severer.Different vegetables had different absorption ability to heavy metals.Zn content was exceeded in every kinds vegetables except Brassica campestris L. ssp. pekinensis(Lour) Olsson.Cu content was exceeded in Lactuca sativa L. var. longifolia Lam.Hg content was exceeded in Apium graveolens L., Lactuca sativa L. var. longifolia Lam. and Spinacia oleracea L.The absorption abilities of Brassica campestris L. ssp. pekinensis(Lour)Olsson for all heavy metals were smaller.The bioconcentration abilities of Lactuca sativa L. var. longifolia Lam., Spinacia oleracea L. Apium graveolens L. and Lactuca sativa L. were generally stronger than other vegetables in this study,and so the edible risk of this three vegetables were bigger too.The health risk of vegetables feeding to children was higher than adults.

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Primary Studies on Inhibitory Effect of Water Extracts from Allium tuberosum on Fusarium oxysporum f. sp. conglutinans in Cabbage
ZENG Feng,HUANG Yong-hong,LING Jian,MAO Zhen-chuan,XIE Bing-yan,YANG Yu-hong*
China Vegetables    2016, 1 (1): 60-63.  
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This paper studied the inhibition role of water extracts from Allium tuberosum on Fusarium oxysporum f. sp. conglutinans(Foc)and control effect of pot cultivation by combining in vitro antibacterial assay with potted plant measurement. The result indicated that extracts could clearly suppress mycelia growth and spore germination.The inhibition rate was up to 100% when the concentration of waterextracts reached 0.75 g·mL-1. The spore germination inhibition rate reached 95.47% when the added leek extract concentration came to 0.25 g·mL-1 and was deal with 20 hours. In pot experiments, the disease control efficiency can reach 40%,and mortality fell by 27% 15 days after the treatment when the concentration of waterextracts reached 0.086 g·g-1 in the culture substrate.

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Identification of Eggplant Resistant to Phomopsis Rot in Hubei Province
YANG Shao-li,WU Ren-feng*,MA Xiao-long,YANG De-zhi
China Vegetables    2016, 1 (1): 64-66.  
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Thirty-four eggplant cultivars were collected from markets in Hubei Province. Their resistance to eggplant phomopsis rot was identified by indoor artificial inoculation in vitro. The results indicated that among 34 cultivars, 8 were immune, 4 were highly resistant,2 were resistant, 2 were middle resistant, 18 were susceptible. Those results showed that more than 50% eggplant varieties extended in Hubei market were susceptible to Phomopsis vexans.

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Evaluation and Analysis of Vegetable Mechanization Level
XIAO Ti-qiong1,2, CHEN Yong-sheng2, HE Chun-xia1*, CUI Si-yuan2
China Vegetables    2016, 1 (1): 67-70.  
Abstract327)      PDF(pc) (1807KB)(1071)       Save

In order to better understand the vegetable mechanization status, regional differences, weak links; scientifically evaluate the vegetable mechanization level, and find out ways for its healthy development, this paper established an evaluation index system for open field vegetable production mechanization based on literature analysis, expert consultation, and spot investigation. This system included 5 secondary indicators, including tillage,planting,harvesting,plant protection and irrigation mechanization level,and provided calculation methods for each indicator. This paper selected 3 typical vegetable gardens in Jiangsu and carried out true case analysis. We found that the evaluation result was consistent with the actual investigation situation. Therefore, this system is of reference significance for making relevant policies to support vegetable industry development, and has provided scientific basis for guiding the development of vegetable mechanization.

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A New Tomato F1 Hybrid with Resistance to TYLCV—‘ Jinongjiafen’
REN Guo-san1,WANG Wen-jun2,CHENG Jia-xiang1,HUANG Chuan-hua1,WANG Xin-feng1,MA Jin-gang3,YAO Fu-you4
China Vegetables    2016, 1 (1): 71-73.  
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‘Jinongjiafen’ is a new tomato F1 Hybrid of mid maturity developed by crossing self-line 09205-7 as female parent and self-line 99011-3 as male parent. The plant has strong growth vigor with high fruit setting ability. It is tolerant to cold. Its fruit is bright pink in color, and of round shape without shoulder. Its fruits are uniform in size with smooth peel. It is tolerant to storage and transportation. It is resistant to TYLCV(containing resistant gene Ty3). The single fruit weight is about 300 g. It can yield about 187.5 t·hm-2. It is suitable to be cultivated in green house in the Northern Yangtze River Valley.

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A New Bitter Gourd F1 Hybrid—‘Xincui’
XIAO Chang-hua,KUANG Bi-feng,YU Xi-mao,DENG Xian-chao,TANG E,TAN Mei-li,LIU Xiaoan,OUYANG Feng
China Vegetables    2016, 1 (1): 73-75.  
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‘Xincui’ is a new bitter gourd F1 Hybrid developed by crossing DH23 as female parent and B08025 as male parent. The plant grows vigorously with outstanding thermal resistance. Its fruit peel is light green in color. Its fruit is of cylindrical shape with protruding tumor,32-35 cm in length and 6-7 cm in width. The fruit flesh is 1.0-1.2 cm in thickness. Its resistances to downy mildew,Fusarium wilt and powdery mildew are stronger than that of the contrast‘ Bilyu No.3’. It is of early maturing. It can sustainably bear fruit,and has obvious high yielding potential. Its yield is over 57 t·hm-2。It is suitable for open and protected field cultivation in Yangtze River Valley and southern China.

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A New Yam Variety —‘Qinfenghuaishan’
CHEN Yang,MA Li-na, ZHOU Xian-zhi,LIN Yong-sheng
China Vegetables    2016, 1 (1): 76-78.  
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The new variety‘ Qinfenghuaishan’is bred by systematic selective method with excellent strains 06-33 collected from local resource material‘ Cunjinshu’ in Quanzhou, Fujian Province. It is of mid and late maturity. The plant has strong growth potential and vigorous branching ability. Its tuber peel is brown in color, and of long cylindrical shape. The fruit is 50-130 cm in length. Its single fruit weight is 500-1 100 g. It tastes good with excellent quality. It can yield about 37.5 t·hm-2. It is suitable to be planted in Fujian Province and the surrounding areas.

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A New Vegetable Soybean Variety —‘ Huaixiandou No.5’
YANG Jia-yin, XU Hai-feng, CHENG Bao-shan, LUO Bo-xiang
China Vegetables    2016, 1 (1): 78-80.  
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‘Huaixiandou No.5’ is a new vegetable spring soybean variety bred by modified single seed descent, and crossing‘ Taiwan 75’ as female parent and‘ Gaoxiong No.5’ as male parent. It yields high, has good quality and is of middle maturity. The average fresh pod yield is 10.05 t·hm-2 and the period from seedling emergence to green pod harvest is 83 days. Its pods are dark green in color with white pubescence. The standard second seed pod is 5.6 cm in length and 1.3 cm in width. The 100 fresh pods weights 312.5 g, and 100 fresh seeds are 77.2 g in weight. It tastes sweet and waxy. It is suitable to be cultivated within Jiangsu Province in spring.

 

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China Vegetables    2016, 1 (1): 81-82.  
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China Vegetables    2016, 1 (1): 83-84.  
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China Vegetables    2016, 1 (1): 85-87.  
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China Vegetables    2016, 1 (1): 88-90.  
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China Vegetables    2016, 1 (1): 91-93.  
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China Vegetables    2016, 1 (1): 94-96.  
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China Vegetables    2016, 1 (1): 96-97.  
Abstract297)      PDF(pc) (1154KB)(1009)       Save
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China Vegetables    2016, 1 (1): 98-100.  
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China Vegetables    2016, 1 (1): 101-103.  
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China Vegetables    2016, 1 (1): 103-104.  
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China Vegetables    2016, 1 (10): 1-4.  
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China Vegetables    2016, 1 (10): 5-9.  
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China Vegetables    2016, 1 (10): 9-12.  
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Analysis of Fruit Vegetable Production in Beijing Based on Micro-perspective of Farmers
KANG Ting,MU Yue-ying
China Vegetables    2016, 1 (10): 13-19.  
Abstract372)      PDF(pc) (2327KB)(1045)       Save

In order to explore the characteristics of fruit vegetable production in Beijing,this study used
descriptive statistical method to analyze the survey data gained from on spots investigation of fruit vegetable farmer
households in Beijing.Then on this base,this paper studies how planting scale,planting technique,circulation
channel,market infrastructure construction,farmer cooperative and supporting policy on vegetable production
affect fruit vegetable production and management.This study also compares the fruit vegetable production in
2012 with that in 2015,so as to study the development and change of fruit vegetable production in Beijing in
recent 4 years from a dynamic perspective.The major conclusions are as following:1)business entity of fruit
vegetable production and management in Beijing is farmer households of small scale,2)there is a remarkable
aging tendency in farmer households engaged in vegetable production,3)there is a growing requirement for new
planting technology,4)the land scale, planting technology,market infrastructure construction and government
policies have significant positive effect on vegetable production,5)government support for fruit vegetable industry
is consistently increasing.

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Research Progress about Effects of Root Zone Temperature on Physiology and Ecology of Protected Horticulture Crops
FU Guo-hai,YANG Qi-chang,LIU Wen-ke,YAN Wen-kai
China Vegetables    2016, 1 (10): 20-27.  
Abstract296)      PDF(pc) (1622KB)(1317)       Save

Horticulture crops grown in protected facilities were more sensitive to root zone temperature than
to air temperature and changes in root zone temperature could cause drastic physiological and ecological changes
in crops. This paper systematically summarized the effects and function machenism of root zone temperature on
physiological ecology of root system and canopy of crops grown in protected facilities. The paper reviewed the
progress made in studying the method and technology for adjusting root zone temperature, the existing problems.
Before ending, it also prospected the future research direction.

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Screening of Tomato Rootstocks with Multi-resistance in Guangxi
JING Zi-huan,WANG Xian-yu,Long An-si,SUN Lan-ming,CHEN Bao-ling
China Vegetables    2016, 1 (10): 28-32.  
Abstract373)      PDF(pc) (1803KB)(1014)       Save

Recently,a mass outbreak of tomato bacterial pith necrosis occurred in Guangxi,except bacterial
wilt,Fusarium wilt and root-knot nematode.This experiment compared and identified 20 pieces of tomato rootstock
material from Guangxi,and screened rootstock material with multi-resistance to tomato bacterial pith necrosis.The
results indicated that rootstocks T034 and T103 were not only resistant to bacterial wilt,Fusarium wilt and root-knot
nematode,but also to bacterial pith necrosis,belonging to multi-resistance polymerization material.

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Accurate Evaluation on Seed Phenotypic Traits of Faba Bean Germplasm Resources
YANG Sheng-hua,LIU Rong,YANG Tao,ZHANG Hong-yan,DU Meng-Ying,ZONG Xu-Xiao
China Vegetables    2016, 1 (10): 32-40.  
Abstract326)      PDF(pc) (1802KB)(1375)       Save

For in-depth understanding the feature and correlation of seed phenotypic traits of faba bean
germplasm resources,comprehensive analyses were conducted in combination with correlation analysis,principal
component analysis and cluster analysis,etc. taking domestic and overseas 554 sets purified for 1 generation
of faba bean germplasm resources by insect nets as material,on seed phenotypic traits obtained from digital
equipment of seed examination. The results revealed that the tested seed phenotypic traits of faba bean germplasm
resources had a wide range variation and rich genetic diversity,especially in the trait of 100-seed weight.
Correlation analysis found that there was high correlation among 6 phenotypic traits of seed,including 100-seed
weight,seed projection area,perimeter,seed length,seed width and diameter;and the correlation coefficients
were greater than 0.80,reaching extremely significant level. The principal component analysis of 8 quantitative
traits showed that the component contribution rate of the former 3 components were 74.60%,23.09% and 1.70%,
respectively,with the cumulative contribution rate of 99.39%,almost covering the whole information of the
quantitative traits of the tested seeds. The variation of the above mentioned 6 phenotypic traits contributed largely to
the first principal component,which were all seed size characters. The cluster analysis showed that all the tested
material were classified into 3 groups associated with sowing time and geographical origin to some extent. Based on
the comprehensive analyses of seed phenotypic traits,11 genotypes with nearly spherical seeds and 8 genotypes
with high yield potential resources were selected for faba bean genetic improvement suitable for mechanized
production,and vegetable faba beans with high yield.

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Studies on Screening Watermelon Varieties with Low Cadmium Accumulation
ZHOU Quan,MA Lu-ping,YI Xue-sai, ZHOU Jian
China Vegetables    2016, 1 (10): 41-43.  
Abstract267)      PDF(pc) (1544KB)(894)       Save

Through multiple spots experiments from 2014-2015, this study carried out test for screening
low cadmium accumulation watermelon varieties on cadmium polluted soil,and analyzed the cadmium contents
in watermelon vine,leaf,root and fruits.The results showed that the average cadmium content in vine was
0.057 0 mg·kg-1,in leaf was 0.062 1 mg·kg-1,in root was 0.054 8 mg·kg-1,in fruit was 0.009 2 mg·kg-1
.The cadmium contents in fruit of tested watermelon varieties were all below GB18406.1—2001 Agricultural
Product Safety and Quality Pollution Free Vegetable Safety Standards(cadmium < 0.05 mg·kg-1).
The cadmium content in different parts of watermelon was leaf > vine > root > fruit.In soil,the cadmium
content was within the range of 0.310-9.480 mg·kg-1.Along with the increase of cadmium content in soil,
cadmium content in watermelon fruit would increase gradually.When the cadmium content in soil was below 9.480
mg·kg-1, the following 8 watermelon varieties‘Xiangxigua No.11’(‘Dongting No.1’),‘Xiangxigua No.19’
(‘Dongting No.3’),‘Jinlihuang’,‘Lyuhu’,‘Mitong’,‘Dongfangjiaozi’,‘Quanxin No.2’,‘Heimiren’
could be used as the optimal selection of cultivated land restoration and planting structure adjustment in cadmium
polluted area.

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Effect of New Type White Sunshade Nets on Seedling Environment and Growth of Tomato Seedlings
LIU Yu-mei,BAI Long-qiang,MU Ying,LI Yan-su
China Vegetables    2016, 1 (10): 44-51.  
Abstract361)      PDF(pc) (3283KB)(1053)       Save

Taking tomato(Lycopersicon esculentum Mill.)cultivar‘Zhongza105’as experimental
material,this paper studied the effects of different sun-shade nets and tranditional black sun-shade nets on
inside light and temperature environment,tomato seedling growth and physiological properties.The result
showed that with the decreasing of shading rate,photon flux density(PFD),air temperature,5 cm and 10
cm soil temperatures all showed an increasing tendency.Compared with the 50% shading rate of black net,the
transmittance of 50% white net was remarkably different.The black sunshade net has higher UV transmittance
rate and there were not much differences between the transmittance rates of infrared light and visible light.While
the transmittance rates of UV light and part of infrard light of white sun-shade net was significantly lower than
the visible light.Therefore,the white sunshade net used as outside shading net of greenhouse could have better
cooling effect.Along with the decreasing shading rate,both black and white net treatments were all helpful for
increasing plant height,stem diameter,leaf area,total biomass,sound seedling index,root system activity and
foliar blade thickness of tomato seedling.Summing up the above,in summer according to the actural condition of
tomato seedling development,sunshade nets with shading rate between 20%-40% might be a good choice,among
them the white sun-shade net with 20% shading rate had the better effect.

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Studies on Stereoscopic Cultivation with Non-woven Fabric as Growing media in Pakchoi
LIU Ping,DU Qing-ping,TANG Peng-xian,XU Shun-fei,WANG Yi-fei
China Vegetables    2016, 1 (10): 52-58.  
Abstract322)      PDF(pc) (3198KB)(837)       Save

Integrative water and fertilizer planting system for pakchoi〔Brassica campestris L. ssp.
chinensis(L.)Makino var. communis Tsen et Lee〕was designed using the non-woven fabric as growing
media.Taking‘Yangzhouqing No.1’and‘Gaogengbai’as material,this experiment studies the effects of
different nutrient solution concentrations on growth and physiological indexes of pakchoi by split block design.
The results indicate that along with the increase of nutrient solution concentrations,the overground fresh weight,
soluble sugar content and chlorophyll SPAD value will firstly increase and then decrease,while the root MDA has
an opposite tendency-firstly decrease and then increase.125% full price nutrition solution treatment is in favour of
upgrading overground fresh weight,soluble sugar content and chlorophyll SPAD value,and maintaining low root
MDA value of pakchoi.

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Temporal Change of CMV and Symptom Phenotype in Pepper after Artificial Inoculation
WANG Xing-xing,ZHANG Zheng-hai,CAO Ya-cong,WANG Li-hao,ZHANG Bao-xi
China Vegetables    2016, 1 (10): 59-62.  
Abstract238)      PDF(pc) (1722KB)(853)       Save

Taking 5 pepper of different genotypes as experimental material,after artificial inoculating heavy
mosaic strain CMV,we tested the temporal change of CMV content in 5,10,15,20,25,30 days by DASELISA.
At the same time,the artificial classification method was used,and we carried out analysis on the
correlation of data gained through these 2 methods.The results showed that the virus content increased at 5,
10,15 days in turn,reached the highest level at 15 days.Then,the virus content reduced slightly.The virus
content of the plants obtained by DAS-ELISA was positively correlated with the disease index obtained by artificial
inoculation.The results obtained by the 2 methods were consistent.

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Studies on Controlling Effect of Bacillus vallismortis NBIF-001 on Gray Mold
HUANG Da-ye,ZHOU Ting,YAO Jing-wu,LIU Xiao-yan,CAO Chun-xia,YANG Ni-na,HU Hong-tao,LONG Tong,YANG Zi-wen
China Vegetables    2016, 1 (10): 63-66.  
Abstract470)      PDF(pc) (2131KB)(1093)       Save

In order to evaluate control effect of Bacillus vallismortis on gray mold of antagonistic bacterium
strain NBIF-001,this paper determined its inhibition activity to the pathogen of Botrytis cinerea by dual culture
method combined with optical microscope observation;and studied its controlling effects on cucumber leaf grey
mold and tomato fruit grey mold by in vitro tissue test.The results showed that the strain NBIF-001 had high
inhibition activity in dual culture.Optical microscope study revealed deformation of hyphal and enlargement
of cytoplasmic vacuoles.In vitro tissue test shew that NBIF-001 exhibited better biological control effect on
cucumber and tomato gray mold.The control effect reached 85.91% and 72.52%, respectively.

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