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| The Establishment and Application of Isolated Microspore Cuilture Technology System in Brassica campestria L.ssp.chinensis var.communis Tsen et lee. |
| Wang Chaonan,Fwng Hui,Jiang Fengying,Liu Rue,Zhou Ying |
| College of Horticulture; Shenyang Agricultural University; Liaoning 110161 |
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Abstract Isolated microspore culture was carried out with ten F1 hybrids of pakchoi(Brassica campestris L.ssp.chinensis var.communis Tsen et Lee.).The frequency of embryogenesis,embryos germinated seedling,subculture and rootage of regenerated plantlets,uniform identification of horticultural characters from the DH line progenies and hybrid made with DH lines were studied.The results showed that genotypes had remarkable effect on embryoid induction.Using semi-content of macro-elements in NLN-13 medium increased the ...
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Received: 11 January 2007
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