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| Morphological Characteristics of Flower Organ and Cytological Observation of Microspore in Different Development Stage in Radish（Raphanus Sativus L.） |
| Li Dan，Li Xixiang，Shen Di |
| （Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081） |
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Abstract The developmental stage of microspores was observed by two different methods： acetic magenta staining and DAPI staining.It was found that the nucleolus in microspore display more clearly by DAPI staining than acetic magenta staining.The relationship between microspore developmental stage and morphological characteristics of flower buds and anthers was studied by both of cytological and morphological methods on four different radish genotypes.There were significant differences in longitudinal diameter and transverse diameter of flower buds，the ratio between longitudinal diameter and transverse diameter of flower buds，anther length，anther width，petal length，the ratio of petal length and anther length，during the developmental stages of the microspore in all four different radish genotypes.At the late-single nucleus stage，significant difference existed in all the above-mentioned index of different varieties.It indicated that the longitudinal diameter of buds，the ratio of the longitudinal diameter and the transverse diameter of flower buds，petal length and the ratio of the petal length and the anther length were suitable for the determination of sampling time for microspore culture.The appropriate longitudinal diameter of buds ranged 2.991-3.510 mm and the ratio of the longitudinal diameter and the transverse diameter of flower bud ranged 1.412-1.480，the petal length ranged 1.889-2.561 mm，the ratio between petal length and anther length ranged 0.923-1.057.In addition，it is necessary that the anthers should be covered by the sepal，the length of sepal was equal or close to the anther lenghth，and the flower bud was full，the anther showed from light yellow to yellow.
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Received: 04 April 2008
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