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| Using Seed Protein SDS-PAGE to Identify Heat-resistant Radish Germplasm |
| ZHU Chang-zhi，LAI De-qiang，WU Bin，ZHANG Yan-yu，LIU Li-wang* |
| National Key Laboratory of Crop Genetics and Germplasm Enhancement，College of Horticulture，Nanjing Agricultural University，Nanjing 210095，Jiangsu，China |
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Abstract Seed proteins of 32 heat-resistant radish（Raphanus sativus L.）accessions were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE）to estimate their polymorphism．A total of 41 protein bands were detected，of which 25（61 %）were polymorphic．Each accession could produce 28 to 39 protein bands，their profiles were different with each other and all accessions could be distinguished．These accessions could be clustered into two major groups of white and red roots at similarity index of 0.76 with UPGMA．The protein band similarity coefficient ranges from 0.64 to 0.98 and the average is 0.79．The results revealed that cluster analysis of seed protein was highly in accordance with radish root color，and low genetic diversity and narrow genetic basis existed in these heat-resistant radish accessions．
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Received: 18 November 2009
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Corresponding Authors:
LIU Li-wang
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