|
|
|
| Studies on Polyphenol Content Determination of Luffa by Folin-Ciocalteu Colorimetry |
HUANG Shu-ping1,TAN Tai-ming1,XU Chang-cheng1,TAN Jie1,LIU Ming1，WANG Meng2
|
| 1Wuhan Vegetable Research Institute,Wuhan 450065,Hubei,China;2College of Horticulture & Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,Hubei,China |
|
|
|
|
Abstract A quantitative determination method for polyphenol content in luffa was studied by using Folin-Ciocalteu colorimetry with gallic acid as standard.The results showed that 2 mL polyphenol contents from luffa could be well calculated according to their colorimetric absorption at 770 nm by applying 2 mL Folin-Ciocalteu reagent,and 4 mL 10 % Na2CO3 for 90 min at 30 ℃ for colorimetric reaction,and the linear range of standard curve was 2-10 mg·L-1.The polyphenol content in luffa could be determined by this method.This method is very stable with good reappearing property and high accuracy.
|
|
Received: 03 September 2009
|
|
|
|
Corresponding Authors:
HUANG Shu-ping
|
|
|
|
|
|
|
