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| Expression of Radish Peroxidase Gene RsPrx1 in Pichia pastoris and Studies on Its Tolerance |
| LI Fang-jun;WANG Li;WANG Yi-fan;SUN Xiang-dong;YANG Jing;HE Hui-hui;CHENG Yuan;WANG Lin-song* |
| College of Life Sciences，Henan Normal University,Xinxiang 453007，Henan,China |
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Abstract RsPrx1 cDNA was amplified by reverse transcription-polymerase chain reaction(RT-PCR)from the peel of Chinese red radish(Raphanus sativus L.) root.The full-length of RsPrx1 cDNA was sequenced.Then it was sub-cloned into Pichia pastoris expression plasmid pPIC9KH.RsPrx1 gene was integrated into the genome of Pichia pastoris and successfully expressed by inducing.The recombinants could show certain resistance to NaCl and H2O2.An efficient expression system of Pichia pastoris was constructed,which was the base of large scale production and functional analysis.
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Received: 13 May 2009
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