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| Radish Germplasm Identification with Isozyme Analysis |
| ZHANG Cui-ping;YUAN Jian-yu;GONG Yi-qin;YANG Jin-lan;WANG Long-zhi;LIU Li-wang* |
| National Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China |
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Abstract Four isozymes:esterase(EST),peroxidase(POD),α-amylase and superoxide dismutase (SOD)extracted from buds of 16 radish(Raphanus sativus L.)accessions were studied with polyacrylamid gel electrophoresis(PAGE).The results showed that a high degree of polymorphism was observed in the EST and POD isozymes.There was a co-dominant locus detected in the zymogram patterns of SOD and α-amylase isozyme,respectively.The isozyme zymogram and cluster analysis revealed genetic diversity and relationship among the tested accessions.This provided valuable theoretical reliance for radish germplasm identification and genetic improvement.
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Received: 01 June 2009
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