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| Analysis of SSR Information in EST Resource of Watermelon(Citrullus lanatus) |
| ZHAO Sheng-jie,LIU Wen-ge*,YAN Zhi-hong,HE Nan |
| Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China |
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Abstract 5 034 non-redundant ESTs of watermelon〔Citrullus lanatus(Thunb.)Matsum.et Nakai〕were screened and 347 SSRs were mined out.The frequency of these EST-SSRs was 689 % and the mean distance was 7.49 kb in non-redundant ESTs.All these SSRs included 91 kinds of repeat motifs.Among them the dinucleotide and trinucleotide repeats were dominant types,accounting for 39.48 % and 46.68 %,respectively.AT is most frequent motif in dinucleotide accounting for 23.35 %.The distribution of trinucleotide repeats was dispersive and the dominant motif was GAA,only accounting for 8.64 % in all the trinucleotide repeats.The EST-SSRs are highly feasible in theory.
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Received: 12 April 2009
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