The effects of genotypes and phytohormone(6-BA)were studied by using cucumber（Cucumis

sativus L.）cotyledon nodes as explant in this experiment.The results showed that genotype was one

of the main influencing factors on shoot regeneration frequency,the cotyledon nodes of different

genotypes cultured in the same medium showed different shoot regeneration frequency.Moreover,6-BA

was essential for the regeneration of cotyledon node.The shoot regeneration frequency of material

228,185,65G increased with the increasing concentration of 6-BA,while the shoot regeneration

frequency of‘Sichunbaigua’,‘Jilinhangua’,K2148 decreased along with the increasing

concentration of 6-BA.The highest shoot regeneration frequency happened on‘Sichuanbaigua’,which

reached 6.4 shoots per-explant cultured in MS1(MS+0.5mg·L^-1 6-BA+2.0 mg·L^-1 AgNO₃)medium.