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| Impacts of Plantlet Age and Cultivation Substrates on Domestication Survival Rate of Transgenic Plantlets of Cucumis sativus L. |
| Miao Minmin，Xu Ran，Zheng Lijuan，et al. |
| （College of Horticulture and Plant Protection，Yangzhou University，Jiangsu 225009） |
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Abstract Taking transgenic plantlets of Cucumis sativus L.as material，we studied the impacts of plantlet age，cultivation substrats and sucrose concentration in rooting media on root development and domestication survival rate of Cucumis sativus L.transgenic in vitro culture plantlets.The results showed that the best root development and the highest survival rate can be obtained when the plantlets were rooted in 1/2 MS media with 6 % sucrose for 28 days and transformed to the same media with 3 % sucrose for 7 days，then transplanted in the cultivation substrates of peat ∶perlite ∶river sand=1 V ∶1 V ∶1 V at 6-true leaf stage.
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Received: 27 March 2008
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