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| In Vitro Culture and Regeneration System Establishment of Pepper |
| LI Haoyu1，2，JIA Li2，SONG Tingting2，WANG Sheng1，2，ZHANG Yu1，2，FANG Ling2，ZHANG Qi’an2，YAN Congsheng2，SUI Yihu1*，JIANG Haikun2* |
| 1Anhui Science and Technology University，Fengyang 233100，Anhui，China；2Institute of Horticulture，Anhui Academy of Agricultural Sciences，Horticultural Crop Germplasm Creation and Physiological Ecology Key Laboratory of Anhui Province，Key Laboratory for Germplasm Creation and Utilization of Horticultural Crops，Ministty of Agriculture and Rural Affairs（Ministry-Province Joint Construction），Hefei 230031，Anhui，China |
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Abstract In order to establish an efficient in vitro regeneration system for chili peppers，five different genotypes of aseptic chili peppers were selected，and the effects of the addition of MS medium with different hormone ratios on bud induction and differentiation，elongation and proliferation，root growth and transplantation were investigated．The results showed that the combination of MS + 6.0 mol·L-1 6-BA + 1.0 mol·L-1 IAA + 5.0 mol·L-1 AgNO3 was the optimal medium for bud differentiation，which could induce white fluffy healing tissues，with a 100.0% induction rate of Flamingo-bill and 86.7% of cotyledons．MS + 3.0 mol·L-1 6-BA + 2.0 mol·L-1 IAA + 5.0 mol·L-1 AgNO3 + 1.5 mol·L-1 GA3 was the optimal medium for adventitious bud elongation，with 66.7% ratios of adventitious bud elongation in Flamingo-bill and 30.0% ratios in cotyledons adventitious bud elongation．MS + 0.5 mol·L-1 NAA was the optimal medium for adventitious bud rooting，the rooting rate of both Flamingo-bill and cotyledon adventitious bud was 100.0%．Regenerated plants were obtained from both Flamingo-bill and cotyledon explants.
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Received: 27 July 2023
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