|
|
|
| Isolation，Identififi cation of Common Bean Sclerotinia Rot Pathogen and Screening of Its Control Fungicide |
| LI Menghua，LIU Dajun，LIU Chang，YANG Xiaoxu，YAN Zhishan，FENG Guojun |
| College of Advanced Agriculture and Ecological Environment，Heilongjiang University，Harbin 150081，Heilongjiang，China |
|
|
|
|
Abstract Abstract：In order to clarify the pathogenic bacteria species of common bean sclerotinia rot in Heilongjiang Province，this paper took bacteria train LMH-001 obtained from common bean sclerotium disease strain as test material；detected its pathogenicity；conducted classification and identification，combined with its morphological characteristics and ITS sequence analysis．This paper also screened bactericides with inhibitory effect on this pathogen through indoor toxicity test．The result showed that pathogenic bacteria for test LMH-001 was Sclerotinia sclerotiorum（Lib.）de Bary．The optimum medium for mycelial growth and sclerotial formation was PDA，temperature was 20-25 ℃，pH was 6，carbon source was glucose，and nitrogen source was yeast powder．Among 11 kinds of fungicide，30% oximidin pentazolol suspension had the strongest inhibitory effect．Its EC50 value was 0.116 9μg·L-1．It could be used as agent of choice for preventing and controlling common bean sclerotium disease．The 29% pentazole and pyrimidin suspension，25% pyrazolesterin suspension，and 40% sclerotium net wettable powder also had better inhibitory eff ects．These 4 fungicides could be used interchangeably．
|
|
Received: 21 November 2022
|
|
|
|
|
|
|
