1Horticultural Research Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007，Guangxi，China；2Vegetable Research Institute，Beijing Academy of Agricultural and Forestry Sciences，Beijing 100097，China；3Sanya Research Institute，Hainan Academy of Agricultural Sciences，Sanya 572000，Hainan，China
Abstract In order to verify the main pathogenic species and hazardous situation of melon virus disease in winter and spring at Hainan region，this paper collected 34 melon samples suspiciously infected by virus disease from main melon producing area during 2022—2023；identified and analyzed the type of melon virus disease pathogen by high-throughput sequencing and polymerase chain reaction（PCR）technology．Results showed that melon yellow spot virus（MYSV），watermelon silver mottle virus（WSMoV）and squash leaf curl China virus（SLCCNV）were prevalent in multiple melon producing areas in Hainan．Among them，the detection rate of MYSV was the highest，being 3.53%，followed by WSMoV with 61.76% detection rate．Moreover，MYSV and WSMoV presented compound infestation phenomenon，with 47.06% mixed infection rate．
