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| Studies on Classification，Identification and Biological Characterization of Chinese Chive Gray Mold Cinerea in Liaoning Province |
| CHEN Le，MIAO Zeyan，SUN Baixin，ZHAO Yang* |
| （Institute of Plant Protection，Liaoning Academy of Agricultural Sciences，Shenyang 110866，Liaoning，China） |
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Abstract Taking the wet-rot type Chinese chives gray mold collected from Beizhen City of Liaoning Province as research object，this paper identified the pathogen by morphological feature and molecular biological method，and studied its biological characteristics．The results showed that the pathogen of Chinese chives gray mold was Botrytis squamosa．The optimum carbon source for mycelia growth was glucose，and suitable nitrogen source was beef extract，yeast extract and peptone．The optimum growth temperature was 25 ℃，and the optium pH was 6.0．The mycelium could grow under the condition of 5.0-11.0 pH and 10-30 ℃．Mycelium would grow slowly when the temperature was over 30 ℃ and stop growing at 35 ℃．The sclerotium generation was sensitive to pH and light．The 5.0-6.0 pH，12 h/12 h alternating light and dark culture were both conductive to the generation of sclerotia．When pH was greater than 6.0 plus 24 h continuous illumination could inhibit the production of sclerotia．
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Received: 24 September 2022
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