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Detection of Pepper Spotted Wilt Virus in Gansu Province |
CHEN Lingzhi1,WANG Lanlan1,ZHANG Ru1,ZHANG Yuxin1,GAO Yanping2 |
1Institute of Vegetables,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China;2Gansu Potato Seed(Seedling)Virus Detection and Safety Evaluation of Engineering Technology Research Center,Lanzhou 730070,Gansu,China |
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Abstract Using RT-PCR methods and simple test strip detection,this study detected pepper pathogenic virus in disease infected samples with symptoms of plant dwarfing,leaf yellowing and growth point necrosis collected from Lanzhou,Baiyin and Qingyang areas of Gansu Province.The results showed that 6 viruses were detected by RT-PCR:chilli ringspot virus(ChiRSV),pepper mild mottle virus(PMMoV),pepper mottle virus(PepMoV),pepper vein yellows virus(PeVYV),tobacco mild green mosaic virus(TMGMV),tomato spotted wilt virus(TSWV).There were multiple virus infection status on the same sample.Simple test strips were used for further detection,only TSWV were detected.But the detection rate was high,indicating that there were many kinds viruses infected in pepper production in Gansu region,and TSWV occurrence was becoming more and more common and serious.
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Received: 23 August 2022
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