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| Genome-wide Identification and Preliminary Exploration on Function of NCED Genes in Garlic（Allium sativum L.） |
| YUAN Xiaoge，LI Fu，ZHANG Xueyu，ZENG Zheng，HE Qiaoyun，ZHOU Zhiman，WANG Yanzhou，YANG Xiai*，LIU Touming* |
| Institute of Bast Fiber Crops，Chinese Academy of Agricultural Sciences，Changsha 410205，Hunan，China |
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Abstract Fourteen AsaNCED genes were identified from the garlic genome using bioinformatics method．It has been predicted that the number of amino acid was 409-599，protein molecular weight were 46 421.95-67 090.27 Da，and theoretical isoelectric point were 5.48-6.87．Exon number of most genes was 1，and minority of them were 11-12．Among the promoter cis-acting elements，the light response elements，Abscisic acid reaction elements and MeJa reaction regulatory elements have an advantage in quantity．They were widely distributed in different gene family members．At the same time，there were drought-related response elements，defense and stress response elements，wound-responsive elements，and seed-specific regulatory elements．The NCED genes family members had certain variability in different tissues．The transgenic Arabidopsis thaliana was obtained by heterologous overexpression of AsaNCED4，whose plant height was significantly lower than that of the wild type．QRT-PCR analyzed revealed that the AsaNCED4 gene could be induced to be up-regulated by salt stress．
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Received: 20 July 2022
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