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| Screening of Antagonistic Bacteria from Strawberry and Their Biocontrol Effect against Tan-brown Leaf Spot of Strawberry |
| WANG Zhuoni1，2 ☆，QIN Genhong1 ☆，WANG Li1，HOU Hui1，YUAN Hongbo1，ZHOU Houcheng1，GONG Wenfeng2*，TU Hongtao1* |
| 1Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，Zhengzhou 450009，Henan，China；2Tibet Agricultural and Animal Husbandry College，Linzhi 860000，Tibet，China |
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Abstract A total of 183 endophytic bacteria strains and 55 rhizosphere bacteria strains were isolated by tissue separation and dilution separation methods from strawberry root，stem，leaf and rhizosphere soil．Among them，9 strains showing broad-spectrum antagonistic effects against 4 strawberry fungal pathogens，including Pilidium concavum，Botrytis cinerea，Fusarium oxysporum and Alternaria alternate．Combining the results of morphological trait，physiology and biochemistry character，and molecular identification，strains G3-23，J3-20 and G3-29 were identified as Bacillus velezensis；J6-1，J1-4，G2-12，G3-K2，and J1-11 were identified as B. subtilis；G3-20 was identified as B. halotolerans；and G3-17 was identified as B. cereus．Strain G3-23 could secrete protease and cellulase，and had a good control effect on strawberry tan-brown leaf spot caused by P. concavum．Besides，this strain could successfully colonize on strawberry leaf，showing a good biocontrol potential．
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Received: 28 July 2022
Published: 22 September 2022
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