|
|
|
| Screening，Identification of Potato Straw Degrading Bacteria and Measuring of Its Degradation Ability |
| XIU Zhijun1，SONG Yadi1，ZHANG Dongmei1，YANG Chunfang1，ZHANG Xuefeng2，ZHANG Xiaoyu1* |
| 1College of Horticulture and Plant Protection，Inner Mongolia Agriculture University，Hohhot 010020，Inner Mongolia，China；2Civilization Practice Guidance Center in the New Era in Ulanqab of Inner Mongolia，Ulanqab 012000，Inner Mongolia，China |
|
|
|
|
Abstract In order to rapidly degradate potato straw，this experiment took soil samples from potato fields，separated straw-degrading bacteria，carried out preliminary sceening by congo red staining method，and obtained one strain，named WXB10．Under the fermentation condition of 28 ℃ and 180 r · min-1 for 35 days，potato straw degradation rate of this strain could reach 57.73%．The strain WXB10 could produce hemicellulase，endonuclease-β-1，4-glucanase，extraneous-β-1，4-glucanase and β-glucosidase．The hemicellulase activity was the highest 32.17 U · mL-1 after being fermented for 5 days．The endonuclease-β-1，4-glucanase activity was the highest 238.75 U · mL-1 after being fermented for 3 days．Both extraneous-β-1，4-glucanase and β-glucosidase activities reached the maximum value after being fermented for 4 days，reaching 478.48 U · mL-1 and 4.82 U · mL-1，respectively．Combining the results of morphological observation，physiological and biochemical experiment，and molecular biological identification，strain WXB10 was identified as Bacillus velezensis，possessing higher cellulase activity and being able to rapidly degrade potato straw．
|
|
Received: 08 April 2022
|
|
|
|
|
|
|
