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| One Year Two-generation Propagation Tetechnology of Cabbage and Its Application in Male Sterility Transformation |
| WANG Dongmei1，GAO Xiaoxu2，XU Jingxiu1，JIA Jun1 |
| 1Beijing Honor Agriculture Development Co.，Ltd.，Beijing 102200，China；2Miyun District Agriculture Technology Promotion Station of Beijing，Beijing 101500，China |
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Abstract This study simulated the growth conditions required by each stage of cabbage propagation，and shortened the time period required by each stage to the minium．Therefor，the aim of completing two breeding generations in one year was realized．Taking seed becoming seedling as a nodal point，one year was devided into 2 cycles：summer-autumn cycle and winter-spring cycle．The period of summer-autumn cycle was slightly longer than that of winter-spring cycle，due to vernalization and flowering．Cultivating method of exfoliating immature ovules could shorten time duration from pollination to seedling and the best time for exfoliating immature ovules was 45-50 days after pollination．Under the condition of 4-7 ℃ vernalization temperature，almost all cabbage varieties could be vernalized within 60 days，except for varieties with strong winterness．This method was only suitable for cabbage male sterility transfer．Basically，varieties without stronger winterness could breed two generations within one year．With SSR markers assisting selection，the backcross female parent for next generation could be accurately determined．
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Received: 26 January 2022
Published: 21 June 2022
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