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Abstract Garden asparagus（Asparagus officinalis L.）is a dioecious plant．It is crucial to distinguish between gender during breeding process．In order to quickly distinguish the sex（XX，XY and YY）of selfcrossing progenies of asparagus hermaphrodites at seedling stage and screen out the supermale plant（YY male plant），this experiment used the improved filter paper adsorption method（disk assemble method）to extract the genomic DNA of asparagus；and combined using the markers of aspMSE1-xy，aspTDF1-y and aspWIP2/NTT-x to distinguish 3 different sex genotypes of asparagus．The results showed that the concentration of genomic DNA extracted by filter paper disk assemble method was suitable for PCR reaction．AspMSE1-xy marker was a co-dominant marker，with 2 specific DNA fragments of 261 bp and 472 bp on the male plant（s XY，YY），and only one specific DNA fragment of 261 bp on the female plant（XX）．AspTDF1-y marker was Y chain-dominant marker with one specific DNA fragment of 472 bp on the male plants（XY，YY）．AspWIP2/NTT-x marker was an X chain-dominant marker with one specific DNA fragment of 346 bp on male plan（t XY）and female plant（XX）．Using this set of markers to distinguish the sexes of 14 single plant from 48-2 selfcrossing progenies of hermaphroditic plant，this experiment screened out 4 supermale plants.
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Received: 26 January 2022
Published: 05 May 2022
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