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| Inhibitory Effect of Biocontrol Streptomyces sp. Strain PBSH9 on Potato Common Scab and Optimization of Its Metabolites Cultivation Condition |
| WANG Liwei 1 ，WAN Zhongyi 2 ，SONG Yadi 1，XIU Zhijun1，YU Shicheng1，DU Meie3，ZHANG Xiaoyu 1* |
1 College of Horticulture and Plant Protection，Inner Mongolia Agricultural University，Hohhot 010019，Inner Mongolia，
China；2 Biopesticide Engineering Research Centre of Hubei Province，Wuhan 430070，Hubei，China；3 College of Erdos
Ecological Environment Vocational，Erdos 017010，Inner Mongolia，China |
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Abstract This study explored the inhibitory effects of biocontrol Streptomyces sp. PBSH9 on potato
common scab caused by Streptomyces galilaeus（PS1） by plate confrontation method，and optimized the
cultivation conditions of strain PBSH9 by using single factor experiment．The results indicated that PBSH9
had stronger inhibiting effect on PS1．After 3 generations’subculture，its inhibiting effects on PS1 were not
weakened．And the inhibition ratios were all over 70%．PBSH9 metabolites could also inhibit PS1 growth，
and the inhibition rate was 75.21%．PBSH9 and PBSH9 metabolites could lead to cross-linking between
hypha，fracture， aerial hypha shorten，bending to straightened，inflation becoming thicker，spore being
out of shape，sporulation quantity going down．The results of PBSH9 cultivation condition optimization
experiment indicated that put in 7 mL seed solution of biocontrol Streptomyces sp. PBSH9 in each 100 mL of
liquid Gao’s No.1 medium，with 7 pH value，and under 28 ℃ constant temperature cultivation for 9 days，the
inhibiting effect of PBSH9 metabolite was the best．The dimeter of inhibitory zone was 23.67 mm．
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Received: 14 November 2021
Published: 16 February 2022
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