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| Isolation and Identification of Three Strains of Pathogen Causing Konjac Stem Rot and Studies on Pathogenicity |
| ZHAO Xingli1，HE Shengling1，LIU Sirui1，LUO Linli1，ZHOU Luona1，FAN Shijie1，ZHOU Yufeng 2* |
1Institute of Biotechnology，Guizhou Academy of Agricultural Sciences，Guiyang 550006，Guizhou，China；2 Tea Research
Institute，Guizhou Academy of Agricultural Sciences，Guiyang 550006，Guizhou，China |
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Abstract Konjac stem rot caused by Fusarium spp. is one of the important soil-borne diseases during
konjac cultivation．In order to clarify the pathogens causing konjac stem rot，this experiment collected several
konjac sickened plant samples from Xingyi City，Guizhou Province in 2019；carried out isolation on these
samples by tissue separation method；identified the isolates by using morphological identification method
combined with molecular method based on rDNA-ITS and tef sequences；then proceeded pathogenicity test
according to Koch’s Postulates．The results showed that 3 strains of Fusarium pure culture were obtained
through tissue separation method．They were belonged to 2 different species，i.e.，F. oxysporum and F. solani
by identification．Further pathogenicity analysis showed that F. oxysporum xymy-8 had no pathogericity，
F. solani xymy-7 and xymy-9 were both pathogenic，and their pathogenicity was different．Among them，
xymy-7 had stronger pathogenicity than xymy-9．The experimental results had provided theoretical basis for
precise prevention and control of konjac stem rot．
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Received: 28 September 2021
Published: 13 January 2022
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