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| Analysis of Potato Common Scab Population Structure and PAI Pathogenic Gene in Heilongjiang Province |
| ZHANG Xuanzhe，ZHAO Xue，CHEN Suhui，XU Haoran，REN Xueqi，ZHU Erlong |
| College of Agriculture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China |
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Abstract This study collected potato tubers infected with potato common scab from 6 cities of Heilongjiang Province（Harbin，Jiamusi，Hegang，Suihua，Mudanjiang and Jixi）from 2018-2020；conducted pathogen isolation on potato samples by dilution method；and tested the pathogenicity of the isolated Streptomyces spp. strains by radish seedling method．This study also carried out classification and identification on pathogenic Streptomyces spp. strains according to morphological characteristics，physiological and biochemical traits，combined with 16S rDNA sequences； and analyzed further the PAI pathogenic gene
composition of pathogenic strains．The results showed that there were 5 pathogenic Streptomyces species in 263 pathogenic Streptomyces strains isolated and identified from 154 potato samples collected in 6 cities，including S. scabies，S. acidicabies，S. bottropensis，S. rochei and S. lavendulae．Among them，S. scabies was the dominant strain，S. bottropensis，S. rochei and S. lavendulae were reported as potato common scab pathogen for the first time in Heilongjiang Province．Through conducting PCR amplification on pathogenicity island genes（txtAB，tomA，nec1）of pathogenic Streptomyces spp.，2 types of disease-causing gene，txtAB+/tomA+/nec1+ and txtAB-/tomA+/nec1+ were detected.
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Received: 20 September 2021
Published: 02 December 2021
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