|
|
|
| Identification of Different Brassica oleracea Vegetables Resistance to Clubroot and Molecular Marker-assisted Selection |
| JIANG Shunshun，LIU Caiming，ZHAO Yu，HU Wenhui，WU Jiabing，LI Qinfei，REN Xuesong，SONG Hongyuan，SI Jun* |
| School of Horticulture and Landscape Architecture，Southwest University，Chongqing Key Laboratory of Vegetable Science，Key Laboratory of Southern Mountain Horticulture，Ministry of Education，Chongqing 400715，China |
|
|
|
|
Abstract Clubroot is one of the main diseases of cruciferous vegetables seriously threating its production．Breeding resistant variety is one of the effective methods to control clubroot．This experiment adopted root irrigation and artificial inoculation methods at seedling stage on physiological race of No.4 clubroot；conducted clubroot resistance identification on 29 Brassica oleracea vegetable accessions；and using molecular marker assisted identification using SCAR marker（OA）and SSR marker（B0902）linked to disease resistance gene CRb and CRbKato on materials expressing immune，high resistant，disease resistant and disease tolerant by artificial inoculation identification．The results indicated that among 8 disease-resistant accessions screened by artificial inoculation identification，‘Cr7’showed immunity，‘Lvkang No.9’and‘Qinglianganlan’showed high resistance，‘S148’‘Cr5’and‘Cr6’showed disease resistance，‘S146’and‘S296’showed disease tolerance．Results of molecular marker identification showed that 4 accessions contained CRb gene，8 accessions contained CRbKato gene，verifying the authenticity of these 8 disease-resistant accessions．Moreover，‘Cr5’‘Lvkang No.9’‘Qinglianganlan’‘S296’accessions contained 2 resistant loci．Therefore，these 4 accessions could be directly applied to breeding of clubroot resistant cabbage．
|
|
Received: 09 September 2021
|
|
|
|
|
|
|
