In order to definite the pathogenic bacteria of strawberry root rot（purple red leaf），recently
occured in Fujian Province，and guide the prevention and control of this disease，this study collected 20
samples from various regions of Fujian Province，carried out isolation and purification，and obtained 5
bacterial strains．Based on the morphological characteristics of these strains and their conidia，the 5 bacterial
strains were identified as Pestalotiopsis sp.．The rDNA-ITS sequences of these strains were amplified by
selected fungus common primer ITS1/ITS4．The ITS sequences of these 5 strains were the same．Through
homology comparison with NCBI database and phylogenetic tree analysis，we found this sequence had the
highest homology with Pestalotiopsis clavispora（Accession：FJ517545.1）．The isolated strains were
inoculated back to healthy strawberry plants．They showed the same disease symptom as in the fields．Same
pathogen could be obtained by re-isolation．The pathogenic bacteria of strawberry root rot in Fujian Province
was identified as Pestalotiopsis clavispora by analyzing morphological character of bacteria strains，genome
ITS sequence analysis and inoculation test．