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| Molecular Identity Card Establishment for Broccoli（Brassica oleracea L. var. italica Plenck）Accessions Using SSR Markers |
| GUAN Junjiao 1，YANG Long 2，MU Wanfu 2，YANG Xiaohong1，ZHANG Peng1，HUANG Qingmei1 ，ZHANG Jianhua 3* |
1Quanlity Standard and Testing Technology Research Institute，Yunnan Academy of Agriculture Sciences，Kunming 650205，
Yunnan，China；2Tropical Eco-agriculture Research Institute，Yunnan Academy of Agriculture Sciences，Yuanmo 651300，
Yunnan，China；3Food Crops Research Institute，Yunnan Academy of Agriculture Sciences，Kunming 650205，Yunnan，
China |
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Abstract In order to rapidly identify and utilize broccoli germplasm resources，taking SSR molecular
marker fluorescence detection technique，this experiment conducted allelic diversity and clustering analysis
on 22 broccoli main cultivated varieties；screened out the best prime combination；and established
molecular identity card（ID）for these 22 broccoli varieties．According to the number of alleles amplified
and polymorphism information content（PIC），this experiment gradually increased the number of primers
starting with one pair of primers，and screened the primer combinations being capable to distinguish all testing
varieties．Finally，4 pairs of core primer were determined，including BoGMS0501，BoE718，BoGMS0941
and BoE723．Digital assignment was conducted on 4 SSR markers in allelic variation in a variety to
establish molecular identity card for 22 broccoli varieties．It is a simple and practical way to establish
molecular identity card for broccoli accessions using SSR marker．The accessions information is concise and
straightforward．This method has provided technical support for authenticity identification and cultivar-rightprotection
of broccoli accessions．
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Received: 28 April 2021
Published: 10 September 2021
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