|
|
|
| Analysis of Genetic Diversity in White Radish Inbred Lines by SSR Marker |
| WANG Qingbiao，WANG Yanping，ZHANG Li* |
Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Key Laboratory of Biology and
Genetic Improvement of Horticultural Crops（North China），Ministry of Agriculture and Rural Affairs，Beijing Key Laboratory
of Vegetable Germplasm Improvement，Beijing 100097，China |
|
|
|
Abstract In order to verify the genetic background of white radish parent and improve the utilization
effeciency of radish breeding material，this experiment carried out genetic diversity analysis on 42 inbred lines
of white radish，using 38 pairs SSR core primer．The results indicated that the number of primers contained
in chromosomes 1-9 were 2，3，4，6，9，2，3，6，3，respectively．One-hundred and twenty-three
allelic variations were detected among 38 pair primers．The average allelic variation number per locus was
2.86，variating from 2-5．The variation amplitude of polymorphism information content（PIC）was 0.21-
0.70．Average figure was 0.52．Among 42 white radish inbred lines，allele numbers of RSS2114 reached
maximum-5．PIC value was 0.68，showing better primer polymorphism and variety discrimination ability．
Hierarchical clustering analysis indicated that the variation range of genetic similarity coefficient between 42
white radish inbred lines was 0.58-1.00．When the genetic similarity coefficient was 0.61，these 42 materials
could be devided into 3 groups．Group 2 could be devided into 5 sub-groups，and group 3 into 2 sub-groups．
Agronomic traits analysis was conducted on the hybrid progenies from the same or different groups．It was
discovered that the yield superiority of cross between group was significantly higher than that within group．
Besides，the genetic relationship of inbred line with tolerance to bolting was closer．It is required to introduce
new germplasm resources to expand the genetic background of this type parent．
|
|
Received: 22 January 2021
Published: 06 April 2021
|
|
|
|
|
|
|
