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| Studies on Tolerance of Different Resistant Tomato yellow leaf curl virus Genes to Tomato chlorosis virus |
| FAN Lina1，GAO Wenzheng1，LI Xiaodong2，HUANG Zejun1，GUO Yanmei1，SHU Jinshuai1，LIU#br# Lei1，LI Junming1，DU Yongchen1，WANG Xiaoxuan 1* |
1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Shaanxi Jinpeng
Seed Co.，Ltd.，Yangling 712100，Shaanxi，China |
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Abstract Tomato yellow leaf curl virus（TYLCV）and Tomato chlorosis virus（ToCV）are the 2
viruses，harming tomato more serious in recent years．This experiment made use of 11 tomato materials
carrying different genes resistant to TYLCV；and carried out studies on ToCV tolerance under co-infection of
TYLCV and ToCV．The results showed that there were differences in tolerance to ToCV by tomato materials
carrying different TYLCV resistance genes．Only materials containing single TY-2/ty-2 resistance gene showed
certain tolerance to ToCV，but their plant height and number of fruit set per plant were greatly affected．
However，tomato materials containing TY-1/ty-1，TY-2/ty-2，TY-3/ty-3 resistance genes at the same time
had better resistance to ToCV．Their disease indexes were below 43.5，and their plant growth potential，fruit
setting rate and fruit maturity were less affected．The CP gene of ToCV and DNA-A whole gene of TYLCV
were cloned and analyzed，respectively．The results showed that the CP gene nucleotide sequence of ToCV
at the time of compound infection was over 99% homogeneous with domestic isolates，and DNA-A gene
nucleotide sequence of TYLCV was over 98% homogeneous with domestic isolates，indicating that no new
viral strains occurred in TYLCV and ToCV at the time of compound infection．
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Received: 09 November 2020
Published: 24 February 2021
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