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| Screening of Efficient Insecticide against Invasive Tuta absoluta（Meyrick）and Detection of Its Resistance Gene Mutation |
| WANG Shaoli，SHI Caihua，XU Dandan，ZHANG Youjun |
| Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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Abstract South American tomato leaf miner，Tuta absoluta（Meyrick）is an alien dangerous pest recently invade into China．This study evaluated the lethal effects of 7 insecticides field dose on larva of T. absoluta invaded into Yining County of Xinjiang Autonomous Region by modified insect blade impregnation method; and conducted detection on point mutations of sodium channel kdr gene associated with pyrethroid insecticide in T. absoluta．The results showed that 1.8% abamectin EW，24% chlorfenapyr SC and 5% chlorantraniliprole SC had skyhigh virulence activity for T. absoluta larva with 100% lethality，followed by 5% emamectin benzoate WG and 6% spinetoram SC with over 90% lethality．However，the virulence activity of Bacillus thuringiensis（Bt）for T. absoluta was lower and 4.5% efficient beta-cypermethrin EC had ultra low virulence activities．The mutation frequency of kdr gene mutation points M918T，T929I and L1014F were 30%，75% and 100%，respectively,consistent to the ultra low activities of pyrethroid insecticide．This results have provided scientific guidance for the chemical prevention and control of T. absoluta invaded into Xinjiang Autonomous Region，China．
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Received: 08 January 2021
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