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| Preliminary Screening and Identification of Molecular Markers Related to Sulforaphane Content in Leaf Mustard Based on BSA-SSR |
| LAN Mei，ZHANG Liqin，HU Jingfeng，XU Xuezhong，YANG Hongli，HE Jiangming* |
| Horticultural Research Institute，Yunnan Academy of Agricultural Sciences，Yunnan Branch of National Vegetable Improvement Center，Kunming 650205，Yunnan，China |
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Abstract In order to obtain molecular markers related to sulforaphane content in leaf mustard，this experiment measured the content of sulforaphane in leaf mustard of F2 generation by high performance liquid chromatography（HPLC）；and constructed F2 mixed gene pool with the bulked segregant analysis（BSA）；and conducted PCR amplification using 50 pairs of primers．Two dominant markers SF12 and SF28 were sieved and obtained．SF12 amplified about size 250 bp bands from parent‘Yuanyechuncai’，low sulforaphane content pool（L）and single plant of low sulforaphane content pool．While，SF28 amplified about size 250 bp bands from parent‘Tailesijiqing’，high sulforaphane content pool（H）and single plant of high sulforaphane content pool．SF12 and SF28 correlated with the sulforaphane content in leaf mustard were verified via some single plants of F2 generation．
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Received: 13 March 2020
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