Abstract Abstract：The genetic diversity of 58 onion germplasm resources was analyzed by SRAP and ISSR molecular
marker technology．Nine ISSR primers with better amplification effects were screened out and a total of 105
clear bands were amplified，including 104 bands with polymorphic sites．The ratio of polymorphic bands
was 99%．Eleven SRAP primers with better amplification effects were screened out and a total of 132 clear
bands were amplified，among which 115 were polymorphic loci．The ratio of polymorphic bands was 87%，
indicating 58 accessions of onion germplasm resource with abundant genetic diversity．The results of clustering
analysis based on 2 kinds molecular marker indicated that 58 accessions of onion germplasm resource could be
divided into 4 major categories．The results of these 2 clustering analysis were basically the same，but SRAP
molecular marker technology could detect more genetic locus，so it was the best molecular marker technology
for genetic diversity analysis．
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Received: 24 April 2020
Published: 18 December 2020
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