Abstract Taking advantage of the CAPS marker C2-25 closely linked to the resistant gene Frl to tomato
Fusarium crown and root rot，this paper developed and designed new SCAR markers to quickly detect the
dominant resistant gene Frl．Using C2-25 as the primer，this experiment conducted PCR among the 25 known
phenotypic tomato material through monoclonal sequencing，of which 19 homozygous resistant material all
containing one same resistant sequence，4 homozygous susceptible material all containing one same susceptible
sequence，both 2 heterozyous resistant material containing the above mentioned resistant and susceptible
sequences．According to SNP differences between the resistant and susceptible sequences，we designed
primers and finally found out primer combination R-6F/2R，S-3F/4R linked to Frl gene and produced 370 bp
resistant fragment and 520 bp susceptible fragment．Testing the primer combination among 500 F2 plants，
the genotype of 473 plants were consistent with their phenotype，meaning the accuracy rate was 94.6%．This
SCAR marker was characterized by high accuracy，low cost and simple operation．Therefor，it can be used in
gene Frl marker assisted breeding．
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Received: 11 May 2020
Published: 28 June 2020
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