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| Effects of Exogenous Myo-inositol on Low Temperature Resistance of Cucumber Seedlings |
| MIAO Tiantian，LI Qiang，YU Hongjun，LIU Peng，HAO Jia，JIANG Weijie* |
| Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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Abstract Taking cucumber（Cucumis sativus L.）‘9930’as test material, this paper studied the effect of spaying myo-inositol（MI，0.8 g · L-1）on cucumber plant resistance and expression level of key gene - CsMIOX1（myo-inositol oxygenase gene）under low temperature（6 ℃）．The result indicated that under low temperature condition comparing with the treatment without spraying MI，spraying MI could significantly increase the biomass，decrease the electrolyte leakage rate．Under low temperature stress，the AsA contents of cucumber leaves treated by spraying MI were significantly higher than that of those without spraying MI 12-24 hours after treatment．The AsA content reached the maximum value，12 hours after treatment, which was increased by 131.27% than that of the CK．After 24 hours low temperature treatment，the SOD and POD activities of MI treatment were always higher than that of without MI treatment．After 12-48 hours under low temperature treatment，the H2O2 content of MI treatment was always significantly lower than that of without MI treatment．Until 48 hours，the MDA content in MI treatment was notably lower than that of without MI treatment．By 24 hours after chilling treatment, the contents of soluble sugar and proline of MI treatment were significantly higher than that of without MI treatment by 54.62% and 155.50%，respectively．After spraying MI，the expression level of CsMIOX1 went up significantly．Exogenous MI treatment stimulated the AsA composition．In conclusion，spraying MI can alleviate the damage of low temperature to cucumber，increase AsA content and activity of antioxidant enzyme system under chilling stress，and improve plant cold resistance．
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Received: 02 April 2020
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