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| Isolation and Identification of Tomato Fusarium Wilt and Evaluation of Disease Resistance of Grafted Rootstocks |
| CHU Xin-pei1，LYU Gui-yun1，LU Xiu-yun2，JIA Qiu-ying1，LIANG Yao1，GAO Hong-bo 1* |
1College of Horticulture，Hebei Agricultural University，Collaborative Innovation Center of Vegetable Industry in Hebei，
Baoding 071000，Hebei，China；2Institute of Plant Protection，Hebei Academy of Agricultural and Forestry Sciences，Baoding
071001，Hebei，China |
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Abstract This paper seperated and purified bacterial strains from wilt tomato plants grown in Dingxing
and Feixiang counties with large tomato cultivation areas of Hebei Province．Mophology and molecular
biology methods were adopted to identify the pathogenic bacteria．The wilt resistance of 10 tomato rootstocks
were identified by root dip method．The results showed that both strains isolated from Dingxing and Feixiang
belonged to Fusarium oxysporum f. sp. lycopersici．PCR amplification was conducted using specificity primers
of Fusarium oxysporum f. sp. lycopersici race，It was proved that these 2 bacterial strains were specialized
forms of Fusarium oxysporum f. sp. lycopersici race 3．Three high resistant and 7 resistant rootstock varieties
were screened out．
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Received: 04 December 2019
Published: 06 March 2020
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